Paitian N A, Markossian K A, Nalbandyan R M
Biochem Biophys Res Commun. 1985 Dec 31;133(3):1104-11. doi: 10.1016/0006-291x(85)91250-1.
Nitrite inhibits the oxygen uptake by the system ferrocytochrome c-cytochrome oxidase with Ki = 1.5 mM. In the absence of ferrocytochrome c the oxygen uptake by cytochrome oxidase in the presence of nitrite was observed indicating that the enzyme has some nitrite oxidase activity. Nitrite induces changes in optical difference spectra of cytochrome oxidase and, in particular, the formation of the transient band at 607 nm. The reciprocal relation was observed between the intensity of this band and the rate of the oxygen uptake by cytochrome oxidase. This means that the form of the enzyme with this band does not involved in the nitrite oxidase activity. It is suggested that the nitrite oxidase activity relates to the oxygen binding site rather than the cytochrome c binding site of the enzyme.
亚硝酸盐抑制亚铁细胞色素c - 细胞色素氧化酶系统对氧的摄取，其抑制常数Ki = 1.5 mM。在没有亚铁细胞色素c的情况下，观察到亚硝酸盐存在时细胞色素氧化酶对氧的摄取，这表明该酶具有一定的亚硝酸盐氧化酶活性。亚硝酸盐会引起细胞色素氧化酶光学差光谱的变化，特别是在607 nm处形成瞬态带。观察到该条带的强度与细胞色素氧化酶对氧的摄取速率之间呈反比关系。这意味着具有该条带的酶形式不参与亚硝酸盐氧化酶活性。有人提出，亚硝酸盐氧化酶活性与该酶的氧结合位点而非细胞色素c结合位点有关。
