Pérez Del Río Eduardo, Martinez Miguel Marc, Veciana Jaume, Ratera Imma, Guasch Judith
Institute of Materials Science of Barcelona (ICMAB-CSIC), Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), and Dynamic Biomaterials for Cancer Immunotherapy, Max Planck Partner Group, ICMAB-CSIC, Campus UAB, 08193 Bellaterra, Spain.
ACS Omega. 2018 May 31;3(5):5273-5280. doi: 10.1021/acsomega.8b00521. Epub 2018 May 16.
Adoptive cell therapy, i.e., the extraction, manipulation, and administration of ex vivo generated autologous T cells to patients, is an emerging alternative to regular procedures in cancer treatment. Nevertheless, these personalized treatments require laborious and expensive laboratory procedures that should be alleviated to enable their incorporation into the clinics. With the objective to improve the ex vivo expansion of large amount of specific T cells, we propose the use of three-dimensional (3D) structures during their activation with artificial antigen-presenting cells, thus resembling the natural environment of the secondary lymphoid organs. Thus, the activation, proliferation, and differentiation of T cells have been analyzed when cultured in the presence of two 3D systems, Matrigel and a 3D polystyrene scaffold, showing an increase in cell proliferation compared to standard suspension systems.
过继性细胞疗法，即从患者体内提取、体外处理并回输经体外激活的自体T细胞，是癌症治疗常规方法之外的一种新兴替代方案。然而，这些个性化治疗需要繁琐且昂贵的实验室操作，应予以简化以使其能够应用于临床。为了改善大量特异性T细胞的体外扩增，我们建议在人工抗原呈递细胞激活T细胞的过程中使用三维（3D）结构，从而模拟二级淋巴器官的自然环境。因此，当T细胞在两种3D系统（基质胶和3D聚苯乙烯支架）存在的情况下进行培养时，我们对其激活、增殖和分化进行了分析，结果显示与标准悬浮系统相比，细胞增殖有所增加。
