Sapozhnikova G P, Lozinov A B
Mikrobiologiia. 1978 Jul-Aug;47(4):682-8.
The activity of alcohol dehydrogenase was compared in subcellular fractions of Torulopsis candida grown on glucose ("glucose cells") and on hexadecane ("hexadecane cells"). Two groups of alcohol dehydrogenases (ADH) were found to be present in the cells: (1) soluble ADH located in the cytosol and (2) ADH bound to the membrane structures and located in the mitochondria and other cell structures. Soluble ADH of "glucose cells" actively dehydrated all alcohols tested from C2 to C16. Ln "hexadecane cells", the activity was low or absent. ADH bound to the cell structures dehydrated mainly C5--C16 alcohols; the activity of these ADH was almost by an order of magnitude higher in the "hexadecane cells" as compared to the "glucose cells" (in contrast to soluble ADH). The character of changes in the activity of ADH toward various alcohols in the course of physiological adaptation of the yeast to oxidation of glucose and hexadecane suggests that both soluble and bound ADH contain at least two ADH, one of which is specific to lower alcohols and the other to higher alcohols.
比较了在葡萄糖（“葡萄糖细胞”）和十六烷（“十六烷细胞”）上生长的假丝酵母亚细胞组分中乙醇脱氢酶的活性。发现细胞中存在两组乙醇脱氢酶（ADH）：（1）位于胞质溶胶中的可溶性ADH，以及（2）与膜结构结合并位于线粒体和其他细胞结构中的ADH。“葡萄糖细胞”的可溶性ADH能使从C2到C16的所有测试醇类有效脱水。在“十六烷细胞”中，该活性较低或不存在。与细胞结构结合的ADH主要使C5 - C16醇类脱水；与 “葡萄糖细胞” 相比，这些ADH在 “十六烷细胞” 中的活性几乎高一个数量级（与可溶性ADH相反）。在酵母对葡萄糖和十六烷氧化的生理适应过程中，ADH对各种醇类活性变化的特征表明，可溶性和结合型ADH都至少含有两种ADH，其中一种对低级醇类具有特异性，另一种对高级醇类具有特异性。
