Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, Madrid, Spain.
Department of Immunology, Ophthalmology & ORL, School of Medicine, Complutense University of Madrid, Madrid, Spain.
Alcohol Clin Exp Res. 2018 Oct;42(10):1828-1840. doi: 10.1111/acer.13840. Epub 2018 Aug 9.
Alcoholic liver disease (ALD) is the most common chronic liver disease in the Western world, and it persists at a high prevalence. Understanding the pathophysiology and successful treatment for ALD is closely associated with the suitability of the animal model, which fully reflects all aspects of the pathogenesis and typical histological findings. This study reviews one of the most widely used experimental models of ALD in rodents-the Lieber-DeCarli (LDC) liquid diet. It is an easy, accurate, reliable, and inexpensive model to study the pathogenesis of early stages of ALD. Here, we discuss the historical background and provide an overview of the advantages and disadvantages of the classical LDC as well as modified "second-hit" models. We also provide a comprehensive protocol for the application of the LDC diet to perform it successfully, reliably, and reproducibly in mice.
酒精性肝病（ALD）是西方世界最常见的慢性肝病，其患病率一直居高不下。了解 ALD 的病理生理学和成功治疗方法与动物模型的适用性密切相关，该模型充分反映了发病机制的各个方面和典型的组织学发现。本研究回顾了啮齿动物中最广泛使用的 ALD 实验模型之一——Lieber-DeCarli（LDC）液体饮食。它是一种用于研究 ALD 早期发病机制的简单、准确、可靠且廉价的模型。在这里，我们讨论了其历史背景，并概述了经典 LDC 以及改良的“二次打击”模型的优缺点。我们还提供了一个综合的方案，以成功、可靠且可重复地在小鼠中应用 LDC 饮食。
