Yuan Feng-Ping, Zeng Qing-Dong, Wu Jian-Hui, Wang Qi-Lin, Yang Zu-Jun, Liang Bang-Ping, Kang Zhen-Sheng, Chen Xin-Hong, Han De-Jun
College of Agronomy, Northwest A&F University, Yangling, China.
Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, Yangling, China.
Front Plant Sci. 2018 Jul 5;9:968. doi: 10.3389/fpls.2018.00968. eCollection 2018.
Stripe rust caused by f. sp. () is a devastating foliar disease that affects common wheat and barley throughout the world. The reasonable deployment of adult plant resistance (APR) wheat varieties is one of the best methods for controlling this disease. Wheat landraces are valuable resources for identifying the genes/QTLs responsible for disease resistance. Humai 15 is a Chinese spring wheat landrace and it has exhibited adequate levels of APR to the prevalent races in field environments for many years. In this study, a population of 177 recombinant inbred lines (RILs) was derived from Humai 15 × Mingxian 169. After screening based on a 90K chip array using 45 RILs and Kompetitive Allelic Specific PCR marker genotyping for the population of RILs, a major effect QTL in Humai 15 was located on the centromere of chromosome 2B, where it accounted for up to 47.2% of the phenotypic variation. Two other minor QTL genes from Humai 15 were located on chromosome arms 3BS and 4BL. The gene was identified on chromosome arm 7DS in Mingxian 169.
由条形柄锈菌小麦专化型（Puccinia striiformis f. sp. tritici）引起的条锈病是一种毁灭性的叶部病害，在全球范围内影响普通小麦和大麦。合理部署成株抗性（APR）小麦品种是控制这种病害的最佳方法之一。小麦地方品种是鉴定抗病相关基因/QTL的宝贵资源。淮麦15是一个中国春小麦地方品种，多年来在田间环境中对流行小种表现出足够水平的成株抗性。在本研究中，从淮麦15×铭贤169衍生出一个由177个重组自交系（RIL）组成的群体。在用45个RIL基于90K芯片阵列进行筛选并对RIL群体进行竞争性等位基因特异性PCR标记基因分型后，淮麦15中的一个主效QTL位于2B染色体的着丝粒上，在那里它占表型变异的47.2%。来自淮麦15的另外两个微效QTL基因位于3BS和4BL染色体臂上。在铭贤169的7DS染色体臂上鉴定出了该基因。
