Zhou Xinli, Li Xin, Han Dejun, Yang Suizhuang, Kang Zhensheng, Ren Runsheng
School of Life Sciences and Engineering, Wheat Research Institute, Southwest University of Science and Technology, Mianyang, China.
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Xianyang, China.
Front Plant Sci. 2022 Jul 6;13:932762. doi: 10.3389/fpls.2022.932762. eCollection 2022.
Winter wheat cultivar Pindong 34 has both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust, which is caused by f. sp. (). To map the quantitative trait loci (QTL) for stripe rust resistance, an F recombinant inbred line (RIL) population from a cross of Mingxian 169 × Pingdong 34 was phenotyped for stripe rust response over multiple years in fields under natural infection conditions and with selected races under controlled greenhouse conditions, and genotyping was performed with a 90K single nucleotide polymorphism (SNP) array chip. Inclusive composite interval mapping (ICIM) identified 12 APR resistance QTLs and 3 ASR resistance QTLs. Among the 12 APR resistance QTLs, (explaining 9.24-13.33% of the phenotypic variation), (11.41-14.80%), - (11.55-16.10%), (9.39-12.78%), (9.52-16.36%), (9.09-17.0%), and (8.87-11.38%) were more abundant than in the five tested environments and (11.05-12.72%), (9.81-13.05%), (9.69-10.57%), (10.36-12.97%), and (9.54-13.15%) were significant in some of the tests. The three ASR resistance QTLs (9.69-13.58%), (9.49-12.07%), and (16.16%) were detected based on the reactions in the seedlings tested with the CYR34 race. Among the 15 QTLs detected in Pindong 34, the ASR resistance gene mapped on the short arm of chromosome 7A was likely similar to the previously reported QTL in the region. The QTLs identified in the present study and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.
冬小麦品种平冬34对由条形柄锈菌小麦专化型（Puccinia striiformis f. sp. tritici）引起的条锈病具有成株抗性（APR）和全生育期抗性（ASR）。为了定位条锈病抗性的数量性状位点（QTL），对明贤169×平冬34杂交产生的F重组自交系（RIL）群体在自然感染条件下的田间多年以及在可控温室条件下用选定小种进行条锈病反应的表型分析，并使用90K单核苷酸多态性（SNP）阵列芯片进行基因分型。完备复合区间作图（ICIM）鉴定出12个成株抗性QTL和3个全生育期抗性QTL。在这12个成株抗性QTL中，QYr.ndau-2B.1（解释表型变异的9.24 - 13.33%）、QYr.ndau-2B.2（11.41 - 14.80%）、QYr.ndau-2D.1（11.55 - 16.10%）、QYr.ndau-4B.1（9.39 - 12.78%）、QYr.ndau-4B.2（9.52 - 16.36%）、QYr.ndau-6B.1（9.09 - 17.0%）和QYr.ndau-7B.1（8.87 - 11.38%）在五个测试环境中比其他QTL更丰富，而QYr.ndau-2D.2（11.05 - 12.72%）、QYr.ndau-4B.3（9.81 - 13.05%）、QYr.ndau-4D.1（9.69 - 10.57%）、QYr.ndau-6B.2（10.36 - 12.97%）和QYr.ndau-7D.1（9.54 - 13.15%）在一些测试中显著。基于用CYR34小种测试幼苗的反应，检测到3个全生育期抗性QTL，即QASR-2B（9.69 - 13.58%）、QASR-4B（9.49 - 12.07%）和QASR-7A（16.
