Wheat Research Institute, School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA.
Theor Appl Genet. 2024 Oct 23;137(11):255. doi: 10.1007/s00122-024-04760-4.
Two major QTL for resistance to stripe rust were mapped on chromosome 2BL and 4BL in spring wheat PI 660072, and their KASP markers were developed. Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat worldwide. Identifying resistance genes is crucial for developing resistant cultivars to control the disease. Spring wheat PI 660072 (Triticum aestivum) has been identified to possess both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust. To elucidate the genetic basis of the resistance in PI 660072, a mapping population consisting of 211 FF recombinant-inbred lines (RILs) was developed from a cross of PI 660072 with susceptible spring wheat Avocet S. The mapping population was phenotyped for stripe rust responses across five field environments from 2020 to 2022 and genotyped using the 15 K SNP (single nucleotide polymorphism) array to map stripe rust resistance loci. The mapping population was also tested at the seedling stage with predominant Chinese Pst races CYR31, CYR32, CYR34 and PST-YX1-3-1 in the greenhouse. Stripe rust resistance genes were identified using the quantitative trait locus (QTL) mapping approach. Two QTL were identified with QYrPI660072.swust-2BL mapped on the long arm of chromosome 2B for ASR and QYrPI660072.swust-4BL on the long arm of chromosome 4B for APR. To facilitate marker-assisted selection breeding, Kompetitive allele specific PCR (KASP) markers, KASP-1269 for QYrPI660072.swust-2BL and KASP-3209 for QYrPI660072.swust-4BL, were developed. These markers could be used to introgress the effective resistance QTL into new wheat cultivars.
两个春小麦 PI 660072 中抗条锈病的主效 QTL 被定位在 2BL 和 4BL 染色体上，并开发了它们的 KASP 标记。条锈病是由小麦条锈菌（Puccinia striiformis f. sp. tritici，Pst）引起的，是全球小麦最具破坏性的病害之一。鉴定抗病基因对于开发抗条锈病的品种以控制病害至关重要。春小麦 PI 660072（Triticum aestivum）被鉴定具有成株期抗性（APR）和全生育期抗性（ASR）。为了阐明 PI 660072 抗性的遗传基础，从 PI 660072 与感病春小麦 Avocet S 的杂交后代中开发了一个由 211 个 FF 重组自交系（RIL）组成的作图群体。该作图群体在 2020 年至 2022 年的五个田间环境中对条锈病反应进行了表型分析，并使用 15K SNP（单核苷酸多态性）阵列进行了基因型分析，以定位条锈病抗性基因。该作图群体还在温室中使用主要的中国 Pst 菌株 CYR31、CYR32、CYR34 和 PST-YX1-3-1 在幼苗期进行了测试。使用数量性状位点（QTL）作图方法鉴定了条锈病抗性基因。在 2B 染色体的长臂上定位到一个与 ASR 相关的 QTL QYrPI660072.swust-2BL，在 4B 染色体的长臂上定位到一个与 APR 相关的 QTL QYrPI660072.swust-4BL。为了便于标记辅助选择育种，开发了 Kompetitive allele specific PCR（KASP）标记 KASP-1269 用于 QYrPI660072.swust-2BL 和 KASP-3209 用于 QYrPI660072.swust-4BL。这些标记可以用于将有效的抗性 QTL 导入新的小麦品种中。
