Nakayama T, Nishizawa K, Kimura G, Sato C
Exp Cell Res. 1986 Mar;163(1):246-54. doi: 10.1016/0014-4827(86)90577-x.
Intranuclear immunofluorescent staining by monoclonal and polyclonal antibodies against microtubule-associated protein-1 (MAP-1) on SV-3Y1 cells disappeared when the cells were treated with 1 mM db-cAMP and 1 mM theophylline for 20-30 min at 37 degrees C. The nuclear dots of immunofluorescence disappeared and reappeared repeatedly on successive incubation of the cells with and without these drugs. The same phenomenon was induced by treatment of the cells with 6 mM theophylline or 6 mM papaverine which inhibits the cAMP-hydrolysing enzyme. The following results seem to support the hypothesis that cAMP-induced transfer of antigenic molecules from the nucleus to the cytoplasm is mediated by microtubules: Partial staining of the nucleus during the transitional period. Bright staining of the cytoplasm on treated cells in contrast to nuclear staining on control cells. Disappearance of the nuclear staining not only by the monoclonal antibody but also by the polyclonal antibody. Complete prevention of disappearance of nuclear dots induced by these drugs by pretreatment of the cells with colchicine (1 microgram/ml) or colcemid (1 microgram/ml).
当SV - 3Y1细胞在37℃下用1 mM二丁酰环磷腺苷（db - cAMP）和1 mM茶碱处理20 - 30分钟时，用抗微管相关蛋白-1（MAP - 1）的单克隆和多克隆抗体进行的核内免疫荧光染色消失。在细胞先后用这些药物处理和未用这些药物处理的连续孵育过程中，免疫荧光的核点反复消失和重新出现。用6 mM茶碱或6 mM罂粟碱（抑制环磷腺苷水解酶）处理细胞也诱导出相同现象。以下结果似乎支持这样的假说：环磷腺苷诱导的抗原分子从细胞核向细胞质的转移是由微管介导的：过渡期细胞核的部分染色。与对照细胞的核染色相比，处理后细胞的细胞质染色明亮。不仅单克隆抗体而且多克隆抗体都使核染色消失。用秋水仙碱（1微克/毫升）或秋水仙酰胺（1微克/毫升）预处理细胞可完全防止这些药物诱导的核点消失。
