肌肉来源的细胞外基质的特性及其在大鼠腹壁缺损中的应用
Characteristic properties of muscular-derived extracellular matrix and its application in rat abdominal wall defects.
作者信息
Yang Zhi, Song Zhicheng, Nie Xin, Yang Jianjun, Zhu Chenfang, Guo Kaijin, Gu Yan
机构信息
Department of General Surgery, Hernia & Abdominal Wall Surgery Center of Shanghai Jiao Tong University, Shanghai Ninth People's Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, PR China.
Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, PR China.
出版信息
Regen Med. 2018 Jul 1;13(5):503-517. doi: 10.2217/rme-2017-0116. Epub 2018 Jul 20.
AIM
To investigate the behavior of porcine-derived decellularized muscular matrix (DMM) in vitro and compare the performance of this biological mesh with that of acellular dermal matrix from pig in a full abdominal wall defect model.
MATERIALS & METHODS: To describe the in vitro properties of the DMM scaffold with extracellular matrix histological analysis, growth factor quantification and scanning electron microscopy analysis. To compare structural and functional remodeling between acellular dermal matrix and DMM implants in a rodent full abdominal wall defect model.
RESULTS & CONCLUSION: The results demonstrated that cellular components were effectively removed in the DMM scaffold, which also maintained a 3D architecture, biochemical components and strong mechanical properties. In vivo experiments confirm that the DMM mesh could promote remodeling and reconstruction of functional skeletal muscle tissue.
目的
研究猪源脱细胞肌肉基质（DMM）的体外行为，并在全腹壁缺损模型中比较中比较这种生物网片与猪脱细胞真皮基质的性能。
材料与方法
通过细胞外基质组织学分析、生长因子定量和扫描电子显微镜分析来描述DMM支架的体外特性。在啮齿动物全腹壁缺损模型中比较脱细胞真皮基质和DMM植入物之间的结构和功能重塑。
结果与结论
结果表明，DMM支架中的细胞成分被有效去除，同时保持了三维结构、生化成分和强大的力学性能。体内实验证实，DMM网片可促进功能性骨骼肌组织的重塑和重建。
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