School of Engineering, Tokai Univ., Hiratsuka, Kanagawa 259-1292, Japan.
UVSOR Synchrotron, Inst. Molecular Science, Okazaki, Aichi 444-8585, Japan.
Ultramicroscopy. 2018 Nov;194:1-6. doi: 10.1016/j.ultramic.2018.07.001. Epub 2018 Jul 5.
Soft X-ray spectromicroscopy was applied to study the quantitative distribution of DNA and protein in a mammalian chromosome at the spatial resolution of 100 nm. The quantities of DNA and protein were evaluated using 1s-π* transition in the NEXAFS spectra at the nitrogen K absorption edge. DNA was not uniformly distributed in the chromosome and DNA/protein ratio was less than 0.497. The present analysis revealed the clues to identify other molecules that contribute to the absorption spectrum of the sample. The results suggested that accumulation of the absorption spectra of relevant molecules would support the refinement of the analysis.
软 X 射线光谱显微镜被应用于在 100nm 的空间分辨率下研究哺乳动物染色体中 DNA 和蛋白质的定量分布。利用氮 K 吸收边缘的近边 EXAFS 谱中的 1s-π*跃迁，评估了 DNA 和蛋白质的数量。DNA 在染色体中分布不均匀，DNA/蛋白质比值小于 0.497。本分析揭示了识别其他分子对样品吸收光谱有贡献的线索。结果表明，相关分子吸收光谱的积累将支持分析的细化。
