School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan.
UVSOR Synchrotron, Institute for Molecular Science, Okazaki, Aichi, Japan.
J Xray Sci Technol. 2018;26(6):877-884. doi: 10.3233/XST-180392.
Soft X-ray spectromicroscopy was applied to study the distribution of DNA and RNA in a mammalian cell at the spatial resolution of 400 nm. The relative distribution of DNA and RNA was examined by the SVD (singular value decomposition) method in aXis2000 program using combined full spectra of DNA and RNA at the absorption edge regions of carbon, nitrogen and oxygen. The absorption of nucleic acid was evaluated using 1s-π* transitions in the NEXAFS spectra at the nitrogen K absorption edge and distributed to DNA and RNA according to the relative level obtained above. The present results revealed the usefulness of the SVD method to discriminate closely related molecules such as DNA and RNA.
软 X 射线光谱显微镜被应用于在 400nm 的空间分辨率下研究哺乳动物细胞中 DNA 和 RNA 的分布。通过使用 Axis2000 程序中的 SVD（奇异值分解）方法，对在碳、氮和氧的吸收边缘区域的 DNA 和 RNA 的组合全谱，研究了 DNA 和 RNA 的相对分布。通过氮 K 吸收边缘的 NEXAFS 谱中的 1s-π*跃迁，评估了核酸的吸收，并根据上述获得的相对水平将其分配给 DNA 和 RNA。本结果表明 SVD 方法可用于区分 DNA 和 RNA 等密切相关的分子。
