State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Environ Pollut. 2018 Oct;241:1098-1105. doi: 10.1016/j.envpol.2018.06.050. Epub 2018 Jun 19.
Tetracyclines and sulfonamides are the two classes of antibiotics commonly used in the medical, industrial and agricultural activities. Their extensive usage has caused the proliferation and propagation of resistant bacteria (ARB) and resistance genes (ARGs) in the environment. In this study, the occurrence and distribution of tetracyclines (TC, OTC and CTC) and sulfonamides (SMX, SCX and TMP), their associated ARB and ARGs were quantified in water and sediments collected from the mainstream of Liaohe River, northeast China. The average concentration of tetracyclines was higher in May, while the concentration of sulfonamides was slightly higher in October. The highest concentrations of the total tetracyclines and sulfonamides in sediments were 2.7×10 ng/g and 2.1×10 ng/g respectively detected in May. All detected ARGs were found generally with high abundance. The tetA, tetB and tetE genes were dominant (4.4×10 to 9.8×10 copies of tet genes/copies of 16S rRNA genes) in total communities, and the average abundance of sul genes was expressed above 10 in the water samples in May and October. Redundance analysis (RDA) and principle component analysis (PCA) indicated that the antibiotic residue was the most important contributor to the level of tetracycline and sulfonamide resistance genes, and some hydrogeological conditions (e.g. flow rate, intersection settlement) influenced the distribution of resistance genes. Results from this study could help understand the proliferation and propagation of antibiotic resistance on a river catchment scale and mitigate the potential risks to public health.
四环素类和磺胺类抗生素是医疗、工业和农业活动中常用的两类抗生素。它们的广泛使用导致了环境中耐药细菌（ARB）和耐药基因（ARGs）的增殖和传播。在本研究中，定量测定了来自中国东北辽河干流的水和沉积物中四环素（TC、OTC 和 CTC）和磺胺类（SMX、SCX 和 TMP）及其相关的 ARB 和 ARGs 的发生和分布情况。四环素的平均浓度在 5 月较高，而磺胺类的浓度在 10 月略高。5 月检测到的沉积物中总四环素和磺胺类的最高浓度分别为 2.7×10 ng/g 和 2.1×10 ng/g。所有检测到的 ARGs 普遍丰度较高。tetA、tetB 和 tetE 基因在总群落中占主导地位（4.4×10 到 9.8×10 拷贝的 tet 基因/16S rRNA 基因拷贝），5 月和 10 月水样中 sul 基因的平均丰度均在 10 以上。冗余分析（RDA）和主成分分析（PCA）表明，抗生素残留是影响四环素和磺胺类耐药基因水平的最重要因素，一些水文地质条件（如流速、交叉口沉降）影响了耐药基因的分布。本研究结果有助于了解流域尺度上抗生素耐药性的增殖和传播，并减轻对公众健康的潜在风险。
