Oudshoorn-Snoek M, Demant P
Int J Cancer. 1986 Feb 15;37(2):303-10. doi: 10.1002/ijc.2910370220.
Thymocytes of leukemic AKR mice were analysed with a fluorescence-activated cell sorter (FACS) using monoclonal antibodies (MAbs) reactive with H-2Kk and H-2Dk and conventional hetero-antibodies against MuLV gp-70 antigens. Comparison was made with thymocytes of the low-leukemia H-2k strain C3H, as well as with thymocytes of young AKR mice, late preleukemic AKR mice (6-9 months old) and mitogen-stimulated thymocytes of young AKR mice. Expression of H-2 antigens increased on cells of the majority of tumors, on thymocytes of some late preleukemic mice and on mitogen-stimulated thymocytes. An increase in H-2K and H-2D antigens was noted: imbalance of the K/D ratio in favor of H-2D was observed mostly in tumors with relatively lower total amounts of H-2K and D antigens; ratio shifts in favor of H-2K were also found, mostly in tumors with relatively higher amounts of H-2K and D antigens. Increased expression of MuLV antigens was found on cells of all tumors and on thymocytes of several late preleukemic mice. We show that in primary spontaneous AKR leukemias, in spite of large individual differences, the expression of high amounts of MuLV gp70 is not random, but is associated with high expression of H-2K and H-2D antigens. The same phenomenon is seen in thymocytes of preleukemic mice, but in mitogen-stimulated thymus cells increase of H-2 expression is not accompanied by increase of MuLV gp70.
使用与H-2Kk和H-2Dk反应的单克隆抗体（MAb）以及针对MuLV gp-70抗原的传统异源抗体，通过荧光激活细胞分选仪（FACS）分析白血病AKR小鼠的胸腺细胞。将其与低白血病H-2k品系C3H的胸腺细胞、年轻AKR小鼠的胸腺细胞、白血病前期晚期AKR小鼠（6至9个月大）的胸腺细胞以及年轻AKR小鼠的丝裂原刺激胸腺细胞进行比较。大多数肿瘤细胞、一些白血病前期晚期小鼠的胸腺细胞以及丝裂原刺激的胸腺细胞上H-2抗原的表达增加。观察到H-2K和H-2D抗原增加：在H-2K和D抗原总量相对较低的肿瘤中，大多观察到K/D比值失衡有利于H-2D；也发现比值向有利于H-2K的方向转变，大多在H-2K和D抗原含量相对较高的肿瘤中。在所有肿瘤细胞和几只白血病前期晚期小鼠的胸腺细胞上发现MuLV抗原表达增加。我们表明，在原发性自发性AKR白血病中，尽管个体差异很大，但大量MuLV gp70的表达并非随机，而是与H-2K和H-2D抗原的高表达相关。在白血病前期小鼠的胸腺细胞中也观察到相同现象，但在丝裂原刺激的胸腺细胞中，H-2表达增加并未伴随MuLV gp70增加。
