Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), UMR CNRS 5023, Université de Lyon, Université Lyon 1, ENTPE, 6 rue Raphael Dubois, 69622, Villeurbanne, France.
Environ Sci Pollut Res Int. 2018 Sep;25(27):27225-27234. doi: 10.1007/s11356-018-2756-x. Epub 2018 Jul 20.
The impact of long-term exposure (6 months) to highly or slightly polluted sediments on the energy metabolism of an ecosystem engineer (the oligochaete Limnodrilus hoffmeisteri) was investigated in laboratory conditions. We evaluated some mitochondrial parameters (respiratory chain activity and ATP production rate) and the accumulation of anaerobic end-products (lactate, alanine, succinate, and propionate). The sediments were collected from stormwater infiltration basins and presented high levels of heavy metals and polycyclic aromatic hydrocarbons (PAHs). These compounds had been drained by the runoff water on impervious surfaces of urban areas during rainfall events. A decrease in the activity of the mitochondrial electron transport chain was observed in worms exposed to the most polluted sediment. Urban contaminants disrupted both aerobic metabolism and mitochondrial functioning, forcing organisms to shift from aerobic to anaerobic metabolism (which is characteristic of a situation of functional hypoxia). Although L. hoffmeisteri is very tolerant to urban pollutants, long-term exposure to high concentrations can cause disruption in mitochondrial activity and therefore energy production. Finally, this study demonstrated that anaerobic end-products could be used as biomarkers to evaluate the impact of a mixture of urban pollutants on invertebrates.
在实验室条件下，研究了长期（6 个月）暴露于高度或轻度污染沉积物对生态系统工程师（寡毛纲 Limnodrilus hoffmeisteri）的能量代谢的影响。我们评估了一些线粒体参数（呼吸链活性和 ATP 产生率）和厌氧终产物（乳酸、丙氨酸、琥珀酸和丙酸）的积累。这些沉积物是从雨水渗透盆地收集的，含有高水平的重金属和多环芳烃（PAHs）。这些化合物在降雨事件中通过城市不透水表面的径流水被排走。暴露于污染最严重沉积物的蠕虫中，线粒体电子传递链的活性下降。城市污染物破坏了有氧代谢和线粒体功能，迫使生物体从有氧代谢转向无氧代谢（这是功能缺氧的特征）。尽管 L. hoffmeisteri 对城市污染物非常耐受，但长期暴露于高浓度污染物会导致线粒体活性的破坏，从而影响能量的产生。最后，这项研究表明，厌氧终产物可以用作生物标志物来评估城市污染物混合物对无脊椎动物的影响。
