甲醛固定用于流式细胞术及艾滋病相关逆转录病毒灭活的效用

Utility of formaldehyde fixation for flow cytometry and inactivation of the AIDS associated retrovirus.

作者信息

Lifson J D, Sasaki D T, Engleman E G

出版信息

J Immunol Methods. 1986 Jan 22;86(1):143-9. doi: 10.1016/0022-1759(86)90278-4.

Abstract

To maximize safety in the setting of an increasing number of requests for flow cytometric analysis of specimens potentially contaminated with the AIDS retrovirus, we evaluated some commonly used fixatives for their ability to inactivate the infectious potential of the virus. We found that both formaldehyde (0.37% v/v) and paraformaldehyde (0.5% w/v) completely inactivated the infectious activity of both free and cell-associated lymphadenopathy associated virus (LAV), the etiologic agent for the acquired immunodeficiency syndrome (AIDS). Based on encouraging preliminary results we formally evaluated the effect of formaldehyde fixation on flow cytometric parameters. In addition to inactivating LAV, 0.37% formaldehyde in phosphate buffered saline preserved light scatter and fluorescence properties of cells stained with fluorescein isothiocyanate (FITC) and beta-phycoerythrin (PE) conjugated monoclonal antibodies. These findings suggest that formalin fixation may be useful for laboratories performing flow cytometric analysis of specimens potentially contaminated with the AIDS virus.

摘要

为了在对可能被艾滋病逆转录病毒污染的标本进行流式细胞术分析的请求数量不断增加的情况下最大限度地提高安全性，我们评估了一些常用固定剂使病毒感染性失活的能力。我们发现，甲醛（0.37% v/v）和多聚甲醛（0.5% w/v）都能完全灭活游离和细胞相关的淋巴结病相关病毒（LAV，获得性免疫缺陷综合征（AIDS）的病原体）的感染活性。基于令人鼓舞的初步结果，我们正式评估了甲醛固定对流式细胞术参数的影响。除了使LAV失活外，磷酸盐缓冲盐水中的0.37%甲醛还保留了用异硫氰酸荧光素（FITC）和β-藻红蛋白（PE）偶联单克隆抗体染色的细胞的光散射和荧光特性。这些发现表明，福尔马林固定可能对进行可能被艾滋病病毒污染的标本的流式细胞术分析的实验室有用。