Islam D, Bardhan P K, Lindberg A A, Christensson B
Division of Clinical Bacteriology, Karolinska Institute, Huddinge University Hospital, Sweden.
Infect Immun. 1995 Aug;63(8):2941-9. doi: 10.1128/iai.63.8.2941-2949.1995.
Immunophenotypic changes in peripheral blood lymphocytes (T, B, and NK cells) in patients during shigellosis was characterized by using triple-color flow cytometry. Eleven Shigella dysenteriae 1-infected adult patients (SDIP), 11 Shigella flexneri-infected adult patients (SFIP), 15 age- and sex-matched healthy controls from Bangladesh (C-B), and 15 healthy volunteers from Sweden (V-S) were studied. In SDIP and SFIP, a significant increase in the CD45RO+ cells in both CD4+ and CD8+ T cells were seen. We found evidence for sequential T-cell activation, as shown by increased proportions of CD25 and CD4+ cells; HLA-DR and CD38 on CD8+ cells, and CD54 on CD4+ and CD8+ cells. We found differences in the lymphocyte activation and subset patterns related to the infecting Shigella species. Thus, a decrease in CD45 expression was seen in SFIP; this decrease progressed further during the disease. The proportions of NK cells (CD56+ cells) and CD3- CD8+ cells out of the total CD8+ cells were increased in SFIP but not in SDIP. The CD3+ CD8+ CD57+ T-cell subset was significantly lower in SDIP than in C-B. The proportion of B-lymphocyte-expressing activation markers CD80 and CD23 was higher in patients than in C-B. There was a significant increase in the proportion of CD4+ T cells and a significant decrease in the percentages of total B cells, the CD3+ CD8+ CD57+ T-cell subset, and the CD56+ CD16+ NK-cell subset for V-S compared with C-B. Our results indicate that distinct subset changes and activation patterns are elicited in SDIP compared with SFIP and also that the degree of activation is related to disease severity. In addition, a common sequence of cell activation was seen during the disease course. The difference in the subset patterns seen in C-B and V-S may be related to differences in the levels or spectra of infectious agents in the environment.
采用三色流式细胞术对志贺菌病患者外周血淋巴细胞（T细胞、B细胞和NK细胞）的免疫表型变化进行了特征分析。研究了11例感染痢疾志贺菌1型的成年患者（SDIP）、11例感染福氏志贺菌的成年患者（SFIP）、15名来自孟加拉国的年龄和性别匹配的健康对照者（C-B）以及15名来自瑞典的健康志愿者（V-S）。在SDIP和SFIP中，CD4+和CD8+ T细胞中的CD45RO+细胞均显著增加。我们发现了T细胞相继激活的证据，表现为CD25和CD4+细胞比例增加；CD8+细胞上的HLA-DR和CD38以及CD4+和CD8+细胞上的CD54增加。我们发现与感染的志贺菌种类相关的淋巴细胞激活和亚群模式存在差异。因此，在SFIP中观察到CD45表达下降；这种下降在疾病过程中进一步加重。SFIP中NK细胞（CD56+细胞）和CD3-CD8+细胞在总CD8+细胞中的比例增加，而SDIP中未增加。SDIP中CD3+CD8+CD57+ T细胞亚群显著低于C-B。表达激活标志物CD80和CD23的B淋巴细胞比例在患者中高于C-B。与C-B相比，V-S中CD4+ T细胞比例显著增加，总B细胞、CD3+CD8+CD57+ T细胞亚群和CD56+CD16+ NK细胞亚群的百分比显著降低。我们的结果表明，与SFIP相比，SDIP中引发了不同的亚群变化和激活模式，并且激活程度与疾病严重程度相关。此外，在疾病过程中观察到了细胞激活的常见序列。C-B和V-S中观察到的亚群模式差异可能与环境中感染因子的水平或谱的差异有关。
