David Geffen School of Medicine at UCLA, Department of Hematology/Oncology, Los Angeles, USA.
Methods. 2012 Jul;57(3):392-7. doi: 10.1016/j.ymeth.2012.02.002. Epub 2012 Feb 21.
Written standard operating procedures (SOPs) are an important tool to assure that recurring tasks in a laboratory are performed in a consistent manner. When the procedure covered in the SOP involves a high-risk activity such as sorting unfixed cells using a jet-in-air sorter, safety elements are critical components of the document. The details on sort sample handling, sorter set-up, validation, operation, troubleshooting, and maintenance, personal protective equipment (PPE), and operator training, outlined in the SOP are to be based on careful risk assessment of the procedure. This review provides background information on the hazards associated with sorting of unfixed cells and the process used to arrive at the appropriate combination of facility design, instrument placement, safety equipment, and practices to be followed.
书面标准操作规程 (SOP) 是确保实验室中重复任务以一致方式执行的重要工具。当 SOP 中涵盖的程序涉及到高风险活动，如使用喷气式空气分类器对未固定的细胞进行分类时，安全要素是文件的关键组成部分。SOP 中概述的有关分类样本处理、分类器设置、验证、操作、故障排除和维护、个人防护设备 (PPE) 和操作人员培训的详细信息应基于对该程序的仔细风险评估。本审查提供了与未固定细胞分类相关的危害以及用于确定适当的设施设计、仪器放置、安全设备和要遵循的实践组合的过程的背景信息。
