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强化异养反硝化在人工湿地中使用四种固体碳源:反硝化效率和细菌群落结构。

Intensified heterotrophic denitrification in constructed wetlands using four solid carbon sources: Denitrification efficiency and bacterial community structure.

机构信息

College of Environmental Science and Engineering, State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, Shanghai 201620, China.

College of Environmental Science and Engineering, State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, Shanghai 201620, China.

出版信息

Bioresour Technol. 2018 Nov;267:416-425. doi: 10.1016/j.biortech.2018.07.029. Epub 2018 Jul 7.

DOI:10.1016/j.biortech.2018.07.029
PMID:30032055
Abstract

Biodenitrification using solid carbon sources is a cost-effective way for nitrate removal. In the study, wheat straw, cotton, poly(butylene succinate), and newspaper was chosen as the carbon source to compare the denitrification efficiency and bacterial communities in constructed wetlands. Parameters including COD, NO-N, NO-N and total nitrogen (TN) were analyzed. Results indicated that newspaper provided significantly higher NO-N and TN removal efficiency than the other three solid carbon sources in low-temperature condition. Moreover, both newspaper and wheat straw allowed high NO-N and TN removal efficiency in high-temperature condition. According to pyrosequencing analysis, denitrifying bacteria Dechloromonas and Thauera were the predominant genus in the anaerobic zone of CO- (3.92 and 2.35%, respectively), WS- (1.97 and 1.02%, respectively) and NP-CWs (1.71 and 1.31%, respectively). Genus of Levilinea was enriched in NP- (1.02%) and WS-CWs (0.91%). Furthermore, genus Paludibacter (2.69%) and Saccharofermentans (3.14%) showed high relative abundance in WS-CWs.

摘要

利用固体碳源进行生物反硝化是去除硝酸盐的一种经济有效的方法。在本研究中,选择了麦秆、棉花、聚丁二酸丁二醇酯和报纸作为碳源,以比较湿地中反硝化效率和细菌群落。分析了 COD、NO-N、NO-N 和总氮(TN)等参数。结果表明,在低温条件下,报纸提供的 NO-N 和 TN 去除效率明显高于其他三种固体碳源。此外,报纸和麦秆在高温条件下也能保持较高的 NO-N 和 TN 去除效率。根据焦磷酸测序分析,反硝化细菌 Dechloromonas 和 Thauera 是 CO-(分别为 3.92%和 2.35%)、WS-(分别为 1.97%和 1.02%)和 NP-CWs(分别为 1.71%和 1.31%)厌氧区的主要属。属 Levilinea 在 NP-(1.02%)和 WS-CWs(0.91%)中得到了富集。此外,属 Paludibacter(2.69%)和 Saccharofermentans(3.14%)在 WS-CWs 中表现出较高的相对丰度。

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