McDonough S H, Staprans S I, Spector D H
J Virol. 1985 Mar;53(3):711-8. doi: 10.1128/JVI.53.3.711-718.1985.
In this report, we describe the size and kinetics of appearance of RNAs from the long repeat of human cytomegalovirus. The most abundant RNA from this region was a 2.7-kilobase (kb) species that was detected throughout the infection and was most abundant at 27 and 72 h after infection. The 2.7-kb RNA was the only major species detected with a probe that included the terminus of the long repeat and the heterogeneous L-S junction region. Other transcripts were detected with probes from the internal portion of the long repeat, including an immediate-early RNA of 1.3 kb, early and late RNAs of 1.2 kb, and minor late transcripts of 4.4, 3.6, 3.3, and 1.8 kb. S1 nuclease and exonuclease VII protection analyses of RNA from immediate-early, early, midpoint, and late times in the infection indicated that the major 2.7-kb RNA was not spliced and that the RNA mapped within the long repeat, 1.6 kb from the heterogeneous region. No evidence for temporally regulated changes in transcription initiation, splicing, or choice of 3' end of this RNA was observed. Nuclease protection analysis also demonstrated that the second most abundant late RNA from this region, the 1.2-kb species, was not spliced and had the same polarity as the 2.7-kb RNA. The 1.2-kb also mapped entirely within the long repeat, with its 3' terminus 1.7 kb upstream from the 5' terminus of the 2.7-kb RNA.
在本报告中，我们描述了来自人巨细胞病毒长重复序列的RNA的大小及出现动力学。该区域最丰富的RNA是一种2.7千碱基（kb）的分子，在整个感染过程中均可检测到，且在感染后27小时和72小时最为丰富。2.7 kb的RNA是用包含长重复序列末端和异质性L-S连接区的探针检测到的唯一主要分子。用来自长重复序列内部区域的探针检测到了其他转录本，包括一个1.3 kb的立即早期RNA、1.2 kb的早期和晚期RNA，以及4.4、3.6、3.3和1.8 kb的少量晚期转录本。对感染过程中立即早期、早期、中期和晚期的RNA进行S1核酸酶和外切核酸酶VII保护分析表明，主要的2.7 kb RNA未发生剪接，且该RNA定位于长重复序列内，距异质性区域1.6 kb。未观察到该RNA在转录起始、剪接或3'末端选择方面存在时间调控变化的证据。核酸酶保护分析还表明，该区域第二丰富的晚期RNA，即1.2 kb的分子，未发生剪接，且与2.7 kb RNA具有相同的极性。1.2 kb的RNA也完全定位于长重复序列内，其3'末端位于2.7 kb RNA的5'末端上游1.7 kb处。
