Choi Hye Jin, Jeong Tae-Yong, Yoon Hyeokjun, Oh Bo Young, Han Young Sun, Hur Myung Je, Kang Sanghoon, Kim Jong-Guk
Incheon Institute of Health & Environment.
Department of Life Sciences and Biotechnology, Kyungpook National University.
J Gen Appl Microbiol. 2018 Nov 9;64(5):232-239. doi: 10.2323/jgam.2017.12.007. Epub 2018 Jul 20.
Coastal ecosystems, play critical ecological roles of which tidal flats are a significant component of coastal wetlands, such as habitat and nutrient cycling in aquatic biology. Microbial communities in tidal flats are known to play vital roles of self-purification. And the microbial ecology of the sediment is easily affected by human activities and pollution. In this paper, we applied pyrosequencing technology to investigate microbial communities in three different tidal flats (Ganghwa Island, Ongnyeon land region and Yeongjong Island) on the Incheon, Korea peninsula. A total of 16,906 sequences were obtained. We used these sequences to identify the dominant phyla in the three tidal flats: Proteobacteria, Chloroflexi, Actinobacteria, and Bacteroidetes. The composition of the bacterial community of Ganghwa Island and the Ongnyeon region were more similar to each other than they were to the bacterial community of Yeongjong Island. Simpson's dominance index of Yeongjong Island was higher than that of the other regions, and the Shannon diversity index of this region was the lowest. Previous research of samples in these regions indicated that the three tidal flats had similar geochemical characteristics. However, their bacterial communities were rather distinct. This might be because the analysis of microbial communities and physiochemical analysis have different perspectives. Therefore, the pyrosequencing of a bacterial community with physiochemical analysis is recommended as an effective monitoring tool for the comprehensive management of tidal flats.
沿海生态系统发挥着关键的生态作用，其中潮滩是沿海湿地的重要组成部分，例如在水生生物学中提供栖息地和进行养分循环。已知潮滩中的微生物群落发挥着重要的自我净化作用。而且沉积物的微生物生态很容易受到人类活动和污染的影响。在本文中，我们应用焦磷酸测序技术研究了韩国半岛仁川的三个不同潮滩（江华岛、瓮岩陆地地区和永宗岛）中的微生物群落。总共获得了16906个序列。我们利用这些序列确定了三个潮滩中的优势门类：变形菌门、绿弯菌门、放线菌门和拟杆菌门。江华岛和瓮岩地区的细菌群落组成彼此之间比它们与永宗岛的细菌群落组成更为相似。永宗岛的辛普森优势度指数高于其他地区，且该地区的香农多样性指数最低。此前对这些地区样本的研究表明，这三个潮滩具有相似的地球化学特征。然而，它们的细菌群落却相当不同。这可能是因为微生物群落分析和理化分析有不同的视角。因此，建议将细菌群落的焦磷酸测序与理化分析相结合，作为潮滩综合管理的有效监测工具。
