Motayo Babatunde Olanrewaju, Adeniji Johnson Adekunle, Faneye Adedayo Omotayo
Department of Virology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Medical Microbiology Unit, Pathology Department, Federal Medical Centre, Abeokuta, Nigeria.
J Pathog. 2018 Jun 24;2018:8425621. doi: 10.1155/2018/8425621. eCollection 2018.
Rotavirus has been identified as a major cause of gastroenteritis in Nigeria. There is limited information on the intragenotype diversity of Nigerian rotavirus isolates. We therefore investigated the molecular characteristics of some rotavirus gene sequences detected in sewage from Nigeria. Seven sewage samples, out of a total of 68, tested positive for rotavirus RNA (10.3%). Genotype G1P[4] was the most common genotype (5 isolates) and one isolate for genotypes G1P[8] and G3P[6]. Phylogenetic analysis of the partial VP7 gene of 3 G1P[4] isolates analyzed identified them as genotype G1 Lineage 2 along with Chinese strains with 99.1% to 100% amino acid similarity. Amino acid substitutions D-97→E and S-147→D/N were observed within the 7-1a and 7-2 domains of VP7 gene among the study G1P4 isolates in reference to vaccine strain RotaTeq®. Phylogenetic analysis of the G3P[6] study isolate identified it as genotype G3 Lineage 3, forming a monophyletic cluster with 100% bootstrap value with other West African strains G3 isolates. Phylogenetic analysis of GIP[4] VP4 genes identified them as P4 Lineage 5, while 3 NSP4 gene sequences belonged to genotype E1, while 1 belonged to E2. The results from this study represent phylogenetic analysis of partial gene sequences of environmental group A rotavirus (RVA) isolates from Nigeria.
轮状病毒已被确认为尼日利亚肠胃炎的主要病因。关于尼日利亚轮状病毒分离株的基因内型多样性的信息有限。因此，我们调查了在尼日利亚污水中检测到的一些轮状病毒基因序列的分子特征。在总共68份污水样本中，有7份轮状病毒RNA检测呈阳性（10.3%）。G1P[4]基因型是最常见的基因型（5株分离株），G1P[8]和G3P[6]基因型各有1株分离株。对3株G1P[4]分离株的部分VP7基因进行系统发育分析，结果表明它们与中国毒株同属于G1基因型第2分支，氨基酸相似性为99.1%至100%。与疫苗株RotaTeq®相比，在研究的G1P4分离株的VP7基因7-1a和7-2结构域内观察到氨基酸替换D-97→E和S-147→D/N。对G3P[6]研究分离株进行系统发育分析，结果表明它属于G3基因型第III分支，与其他西非G3分离株形成一个自展值为100%的单系簇。对G1P[4] VP4基因进行系统发育分析，结果表明它们属于P4基因型第V分支，而3条NSP4基因序列属于E1基因型，1条属于E2基因型。本研究结果代表了对来自尼日利亚的环境A组轮状病毒（RVA）分离株部分基因序列的系统发育分析。
