Motayo Babatunde O, Faneye Adedayo O, Adeniji Johnson A
Department of Medical Microbiology, Federal Medical Center, Abeokuta, Nigeria.
Department of Virology, University of Ibadan, Ibadan, Nigeria.
Virus Genes. 2022 Jun;58(3):180-187. doi: 10.1007/s11262-022-01895-8. Epub 2022 Mar 18.
Species A rotavirus are an important cause of childhood gastroenteritis, and the main contributor to its pathogenicity is the enterotoxin (NSP4) protein. Some biophysical properties of partial NSP4 genes of RVAs isolated from sewage in Nigeria during 2014/2015 were investigated. Samples were typed by RT-PCR and Sanger sequencing of partial VP4, VP7 and NSP4 genes. Phylogeny identified lineages within genotypes, predicted glycosylation sites; hydrophobicity profiles and amino acid alignments were employed to determine some biophysical properties of the NSP4 protein. The VP7 sequences of our isolates were the most diversified, the majority of the isolates carried NSP4 genes of the E1 genotype. Genotype specific variations both in hydrophobicity and potential glycosylation were identified, mutations were highest within the H3 hydrophobic domain and VP4 binding domain. The study of RVA NSP4 genes from non-clinical samples revealed that there were structural consistencies with those of reference genes.
A 组轮状病毒是儿童肠胃炎的重要病因，其致病性的主要贡献者是肠毒素（NSP4）蛋白。对2014/2015年期间从尼日利亚污水中分离出的轮状病毒A组部分NSP4基因的一些生物物理特性进行了研究。通过RT-PCR以及对部分VP4、VP7和NSP4基因进行桑格测序对样本进行分型。系统发育分析确定了基因型内的谱系，预测了糖基化位点；利用疏水性图谱和氨基酸比对来确定NSP4蛋白的一些生物物理特性。我们分离株的VP7序列最为多样，大多数分离株携带E1基因型的NSP4基因。在疏水性和潜在糖基化方面均鉴定出基因型特异性变异，H3疏水结构域和VP4结合结构域内的突变最多。对来自非临床样本的轮状病毒A组NSP4基因的研究表明，其与参考基因在结构上具有一致性。
