Anderson P, Smith D H
Infect Immun. 1977 Feb;15(2):472-7. doi: 10.1128/iai.15.2.472-477.1977.
The capsular polysaccharide (CP) of Haemophilus influenzae type b is known to be spontaneously released from the cells in culture. The CP is precipitable from culture supernatant by the cationic detergent hexadecyltrimethylammonium. Most of the nucleic acid and some of the protein, but almost none of the endotoxin, in the supernatant are co-precipitated. Extraction of the precipitate with progressively stronger NaCl solutions separates nucleic acid and protein from the CP and also effects a molecular size fractionation. Residual endotoxin and protein can be reduced by extraction with cold phenol and ultracentrifugation. The resulting preparation has ribose, ribitol, and phosphate as principal components and contains less than 1% other sugars, protein, or nucleic acid; it elutes on Sepharose 2B as a symmetrical peak with Kav 0.51.
已知b型流感嗜血杆菌的荚膜多糖（CP）可在培养过程中从细胞中自发释放。CP可被阳离子去污剂十六烷基三甲基铵从培养上清液中沉淀出来。上清液中的大部分核酸和部分蛋白质会被共沉淀，但几乎没有内毒素。用浓度逐渐增加的NaCl溶液提取沉淀物可将核酸和蛋白质与CP分离，同时也能实现分子大小分级。通过冷酚提取和超速离心可减少残留的内毒素和蛋白质。所得制剂的主要成分是核糖、核糖醇和磷酸盐，其他糖类、蛋白质或核酸含量不到1%；它在琼脂糖2B上洗脱时呈现对称峰，分配系数（Kav）为0.51。
