Tsibris J C, Virgin S D, Khan-Dawood F S, Langenberg P W, Thomason J L, Spellacy W N
Obstet Gynecol. 1986 Mar;67(3):316-20.
The specific activity of guaiacol peroxidase was measured daily in human cervical mucus, vaginal fluids, and saliva during 45 cycles in 31 women. Also determined were basal body temperatures and serum hormones (luteinizing hormone [LH], estradiol, progesterone). The guaiacol peroxidase was extracted with 0.5 M CaCl2 and thus may be a different peroxidase from that obtained by noncalcium extraction procedures. The guaiacol peroxidase specific activity did not vary in the saliva during the cycle but fell sharply in the cervical mucus and vaginal fluid four to five days before the ovulation time, estimated by the LH peak, and rose again one to two days after ovulation. Anovulatory cycles did not show the midcycle drop in guaiacol peroxidase. Growth curve analysis gave excellent fitting of the guaiacol peroxidase data to a polynominal model. These data suggest that cervicovaginal guaiacol peroxidase may be clinically useful in detecting the fertile period for population control and for infertility treatment.
在31名女性的45个月经周期中，每天测量其宫颈黏液、阴道分泌物和唾液中愈创木酚过氧化物酶的比活性。同时还测定了基础体温和血清激素（促黄体生成素[LH]、雌二醇、孕酮）。愈创木酚过氧化物酶用0.5M氯化钙提取，因此可能与通过非钙提取程序获得的过氧化物酶不同。愈创木酚过氧化物酶的比活性在月经周期中唾液中无变化，但在排卵前四到五天，根据LH峰值估计，宫颈黏液和阴道分泌物中的该酶比活性急剧下降，排卵后一到两天又再次上升。无排卵周期未显示愈创木酚过氧化物酶在月经周期中期的下降。生长曲线分析表明，愈创木酚过氧化物酶的数据与多项式模型拟合良好。这些数据表明，宫颈阴道愈创木酚过氧化物酶在检测生育期以进行人口控制和不孕症治疗方面可能具有临床应用价值。
