Tsibris J C, Langenberg P W, Khan-Dawood F S, Spellacy W N
Fertil Steril. 1985 Aug;44(2):236-40.
Thirty-one normal women were studied daily in 41 cycles. Venous blood samples were taken for measurements of luteinizing hormone (LH), estradiol (E2), and progesterone (P), and vaginal examinations were done to obtain cervical mucus and vaginal fluid. The specific activity of guaiacol peroxidase (GP), extracted from cervicovaginal secretions with 0.5 M CaCl2, was determined in the vaginal samples. In the follicular phase, from day -7 to day 0 (the LH +1 day, when ovulation presumably occurred), there was a strong negative correlation between GP and the rising E2 (r = -0.94). On days 1 to 10 after ovulation, there was a strong positive correlation between GP and P (r = 0.84). In nine ovulatory cycles in which P levels did not exceed 8 ng/ml on any day, indicating possible luteal phase inadequacy, there were significantly lower GP levels than in another 32 ovulatory cycles with higher P (P = 0.04). These results suggest that (1) at midcycle, E2 seems to "down-regulate" the GP specific activity; and (2) in the luteal phase, serum P levels parallel those of GP activity, even in the presence of high luteal E2. GP activity profiles during the menstrual cycle can be used to define the fertile period, may prove useful in diagnosing pregnancy, and may be a simple, convenient test for an inadequate corpus luteum.
对31名正常女性进行了研究，共涉及41个周期。每天采集静脉血样以测量促黄体生成素（LH）、雌二醇（E2）和孕酮（P），并进行阴道检查以获取宫颈黏液和阴道分泌物。用0.5M氯化钙从宫颈阴道分泌物中提取的愈创木酚过氧化物酶（GP）的比活性在阴道样本中进行测定。在卵泡期，从第-7天到第0天（LH峰值+1天，推测为排卵发生时），GP与升高的E2之间存在强烈的负相关（r=-0.94）。在排卵后的第1至10天，GP与P之间存在强烈的正相关（r=0.84）。在9个排卵周期中，P水平在任何一天都未超过8ng/ml，表明可能存在黄体期缺陷，这些周期中的GP水平明显低于另外32个P水平较高的排卵周期（P=0.04）。这些结果表明：（1）在月经周期中期，E2似乎“下调”了GP的比活性；（2）在黄体期，即使存在高水平的黄体期E2，血清P水平也与GP活性平行。月经周期中的GP活性曲线可用于确定排卵期，可能有助于诊断妊娠，并且可能是一种用于诊断黄体功能不全的简单、便捷的检测方法。
