Page G, Salvato M, Goodman H M
Nucleic Acids Res. 1986 Jan 24;14(2):899-914. doi: 10.1093/nar/14.2.899.
Repetitive DNA sequences have been found within as well as adjacent to the rat growth hormone (rGH) gene (1,2). In vitro transcription of the rGH gene yields transcripts predominantly from a tandemly duplicated repeat (RU) in the second intron of the gene. The relative alpha-amanitin sensitivity of this transcription indicates that it is carried out by RNA polymerase III. Transcription initiates within a 5' flanking 15 base-pair repeat, and terminates within a stretch of A residues at the end of each repeat, a termination site that is notably unlike most of those used by polymerase III. Approximately 75% of the transcripts stop at the end of the first repeat; the remainder "read-through" to a homologous termination region at the end of the second repeat.
在大鼠生长激素（rGH）基因内部及其相邻区域发现了重复DNA序列（1,2）。rGH基因的体外转录主要产生来自该基因第二个内含子中串联重复序列（RU）的转录本。这种转录对α-鹅膏蕈碱的相对敏感性表明它是由RNA聚合酶III进行的。转录起始于5'侧翼的15个碱基对重复序列内，并在每个重复序列末端的一段A残基处终止，该终止位点与聚合酶III使用的大多数终止位点明显不同。大约75%的转录本在第一个重复序列末端停止；其余的“通读”到第二个重复序列末端的同源终止区域。
