a Basic Medical Sciences, Faculty of Pharmacy , International Islamic University Malaysia , Kuantan , Malaysia.
b Basic Medical Sciences , International Islamic University Malaysia , Kuantan , Malaysia.
Nat Prod Res. 2019 Aug;33(15):2266-2270. doi: 10.1080/14786419.2018.1493587. Epub 2018 Jul 24.
The current study provides a way of extraction for both active NSO and WSE from seeds using 98% methanol. About 1 kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72 hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4 °C, NSO and WSE were obtained at the same instant. Solubility tests of 24 solvents and 11 thin layer chromatographic analyses while 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging assay of NSO (73.66) , WSE (33.32) and NSO + WSE (78.22) against ascorbic acid (IC50 = 4.28 mg/mL) was performed. WSE was found to be highly soluble in water and 5% NaOH exhibiting the same R value of 0.95 for EtOH:DMSO (9:1) against the honey. WSE has revealed more than twofold higher anti-oxidant activity than others. Formulation of WSE with honey may provide better targeted hydrophilic drug delivery systems.
本研究提供了一种从种子中提取 NSO 和 WSE 的方法，使用 98%甲醇。将约 1kg 的粉碎种子以 1:2.5 w/v（g/mL）的比例在 4°C 下浸泡 72 小时。旋转蒸发后，在 50°C 和 4°C 下连续干燥和冷却 7 天，即可同时获得 NSO 和 WSE。对 24 种溶剂和 11 种薄层色谱分析的溶解度测试，以及对 NSO（73.66）、WSE（33.32）和 NSO+WSE（78.22）对 2,2-二苯基-1-苦基肼自由基清除活性的测定（IC50=4.28mg/mL）。发现 WSE 在水中和 5%NaOH 中高度溶解，其在 EtOH:DMSO（9:1）中与蜂蜜的 R 值相同，为 0.95。WSE 的抗氧化活性比其他物质高两倍以上。WSE 与蜂蜜的制剂可能为靶向亲水药物传递系统提供更好的选择。
