Effects of Oil Fractions on Reactive Oxygen Species and Chemokine Expression in Airway Smooth Muscle Cells.

BACKGROUND

many previous studies have demonstrated the therapeutic potential of total oil fractions, neutral lipids (NLs), glycolipids (GLs), phospholipids (PLs), and unsaponifiable (IS) in asthma patients. We therefore tested its effect on airway smooth muscle (ASM) cells by observing its ability to regulate the production of glucocorticoid (GC)-insensitive chemokines in cells treated with TNF-α/IFN-γ, and its antioxidative and reactive oxygen species (ROS) scavenging properties.

MATERIALS AND METHODS

the cytotoxicity of oil fractions was assessed using an MTT assay. ASM cells were treated with TNF-α/IFN-γ for 24 h in the presence of different concentrations of oil fractions. An ELISA assay was used to determine the effect of oil fractions on chemokine production (CCL5, CXCL-10, and CXCL-8). The scavenging effect of oil fractions was evaluated on three reactive oxygen species (ROS), O•, OH•, and HO.

RESULTS

our results show that different oil fractions used at 25 and 50 µg/mL did not affect cell viability. All fractions of oil inhibited chemokines in a concentration-dependent manner. Interestingly, the total oil fraction showed the most significant effect of chemokine inhibition, and had the highest percentage of ROS scavenging effect.

CONCLUSION

these results suggest that oil modulates the proinflammatory actions of human ASM cells by inhibiting the production of GC-insensitive chemokines.