Mosbah Asma, Khither Hanane, Mosbah Camélia, Slimani Abdelkader, Mahrouk Abdelkader, Akkal Salah, Nieto Gema
Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Constantine 1, Constantine 25000, Algeria.
Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University of Ferhat Abbas Setif 1, Setif 19000, Algeria.
Plants (Basel). 2023 May 30;12(11):2171. doi: 10.3390/plants12112171.
many previous studies have demonstrated the therapeutic potential of total oil fractions, neutral lipids (NLs), glycolipids (GLs), phospholipids (PLs), and unsaponifiable (IS) in asthma patients. We therefore tested its effect on airway smooth muscle (ASM) cells by observing its ability to regulate the production of glucocorticoid (GC)-insensitive chemokines in cells treated with TNF-α/IFN-γ, and its antioxidative and reactive oxygen species (ROS) scavenging properties.
the cytotoxicity of oil fractions was assessed using an MTT assay. ASM cells were treated with TNF-α/IFN-γ for 24 h in the presence of different concentrations of oil fractions. An ELISA assay was used to determine the effect of oil fractions on chemokine production (CCL5, CXCL-10, and CXCL-8). The scavenging effect of oil fractions was evaluated on three reactive oxygen species (ROS), O•, OH•, and HO.
our results show that different oil fractions used at 25 and 50 µg/mL did not affect cell viability. All fractions of oil inhibited chemokines in a concentration-dependent manner. Interestingly, the total oil fraction showed the most significant effect of chemokine inhibition, and had the highest percentage of ROS scavenging effect.
these results suggest that oil modulates the proinflammatory actions of human ASM cells by inhibiting the production of GC-insensitive chemokines.
许多先前的研究已经证明了全油馏分、中性脂质（NLs）、糖脂（GLs）、磷脂（PLs）和不皂化物（IS）对哮喘患者的治疗潜力。因此，我们通过观察其调节经肿瘤坏死因子-α/干扰素-γ处理的细胞中糖皮质激素（GC）不敏感趋化因子产生的能力及其抗氧化和活性氧（ROS）清除特性，来测试其对气道平滑肌（ASM）细胞的影响。
使用MTT法评估油馏分的细胞毒性。在不同浓度的油馏分存在下，用肿瘤坏死因子-α/干扰素-γ处理ASM细胞24小时。采用酶联免疫吸附测定（ELISA）法确定油馏分对趋化因子产生（CCL5、CXCL-10和CXCL-8）的影响。评估油馏分对三种活性氧（ROS），即超氧阴离子（O•）、羟基自由基（OH•）和过氧化氢（HO）的清除作用。
我们的结果表明，25和50μg/mL的不同油馏分不影响细胞活力。所有油馏分均以浓度依赖的方式抑制趋化因子。有趣的是，全油馏分显示出最显著的趋化因子抑制作用，并且具有最高百分比的ROS清除效果。
这些结果表明，油通过抑制GC不敏感趋化因子的产生来调节人ASM细胞的促炎作用。
