Bankert R B, Mayers G L, Pressman D
J Immunol. 1977 Apr;118(4):1265-70.
The bacterial dextran B1355, which is normally a potent thymus-independent immunogen, was made tolerogenic by oxidation. The injection of the oxidized dextran into BALB/c mice before, at the same time, or up to 4 days after the injection of the immunogenic form of the dextran resulted in a marked immunologically specific suppression of the number of anti-dextran antibody-forming cells found in the spleen. This suppression resulted from a direct inactivation of antibody-forming cell precursors rather than from either inhibition of antibody secretion or the exhaustive utilization of precursor B cells that have been observed in other tolerance systems. A substantial degree of tolerance was achieved after only a 1-hr in vivo exposure of the spleen cells to the tolerogen. At a dose of 1 mg of oxidized dextran per mouse, tolerance persised for at least 3 weeks. A complete recovery was apparent by 10 weeks. The stability of the tolerance was demonstrated by transferring tolerant spleen cells to irradiated recipients. The response in the recipient animals to an immunogenic dextran challenge remained suppressed. It appears that the tolerogenicity of the oxidized dextran is due to its ability to couple covalently with free amino groups in or near the receptor site of the cell membrane via the reactive dialdehyde groups of the dextran.
细菌葡聚糖B1355通常是一种强效的非胸腺依赖性免疫原，经氧化后具有致耐受性。在注射免疫原性形式的葡聚糖之前、同时或之后4天内，将氧化葡聚糖注射到BALB/c小鼠体内，会导致脾脏中抗葡聚糖抗体形成细胞数量出现明显的免疫特异性抑制。这种抑制是由于抗体形成细胞前体的直接失活，而非像在其他耐受系统中观察到的那样，是由于抗体分泌的抑制或前体B细胞的耗尽。仅让脾细胞在体内接触致耐受原1小时后，就实现了相当程度的耐受性。每只小鼠注射1毫克氧化葡聚糖的剂量时，耐受性持续至少3周。到10周时明显完全恢复。通过将耐受脾细胞转移到受辐照的受体动物身上，证明了耐受性的稳定性。受体动物对免疫原性葡聚糖攻击的反应仍然受到抑制。氧化葡聚糖的致耐受性似乎是由于其能够通过葡聚糖的反应性二醛基团与细胞膜受体位点内或附近的游离氨基共价结合。
