Fernandez C, Möller G
J Exp Med. 1977 Jul 1;146(1):308-12. doi: 10.1084/jem.146.1.308.
Mice were rendered specifically tolerant to the fluorescein isothiocyanatedextran (FITC) epitope by injection of FITC-dextran B512. Their spleen cells were removed at various times and cultivated in vitro with different polyclonal B-cell activators, such as lipopolysaccharide (LPS), purified protein derivative of tuberculin, and native dextran. LPS caused the appearance of high affinity anti-FITC plaque-forming cells to an equal extent with cells from untreated and tolerant animals, whereas native dextran failed to activate cells from tolerant mice, although it was a potent activator of normal cells. It was concluded that tolerance induction only affects those B cells that could respond to the polyclonal B-cell-activating properties of the tolerogen, but not other B cells having an identical set of Ig receptors directed against the tolerogen.
通过注射异硫氰酸荧光素葡聚糖（FITC）B512，使小鼠对异硫氰酸荧光素标记的葡聚糖（FITC）表位产生特异性耐受。在不同时间取出它们的脾细胞，并与不同的多克隆B细胞激活剂一起在体外培养，如脂多糖（LPS）、结核菌素纯蛋白衍生物和天然葡聚糖。LPS诱导产生高亲和力抗FITC噬斑形成细胞的程度，与未处理动物和耐受动物的细胞相同，而天然葡聚糖虽然是正常细胞的有效激活剂，但未能激活耐受小鼠的细胞。得出的结论是，耐受性诱导仅影响那些能够对耐受原的多克隆B细胞激活特性作出反应的B细胞，而不影响具有针对耐受原的相同一组Ig受体的其他B细胞。
