Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.
Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.
Exp Eye Res. 2018 Dec;177:1-11. doi: 10.1016/j.exer.2018.07.022. Epub 2018 Jul 21.
To determine the characteristics of the damages of the retinal pigment epithelium (RPE) and photoreceptors of pigmented mice induced by exposure to blue light emitting diode (LED) light, and to determine the mechanisms causing the damages. Exposure to blue LED light for 3 days induced retinal damage, and the characteristics of the damage differed from that induced by white fluorescent light exposure. Ophthalmoscopy showed that blue LED exposure for 3 days induced white spots on the retina, and histological examinations showed materials accumulated at the IS/OS junction of the photoreceptors. The accumulated materials were stained by ionized calcium binding adapter molecule-1 (Iba-1), a marker for macrophages. The debris was also positive for periodic acid-Schiff (PAS). An enlarging the area of RPE was detected just after the blue LED exposure especially around the optic nerve, and this led to a secondary degeneration of the photoreceptors. Exposure of pigmented mice to 3 consecutive days of blue LED light will cause RPE and photoreceptor damage. The damage led to an accumulation of macrophages and drusen-like materials around the outer segments of the photoreceptors. This blue light exposed model may be useful for investigating the pathogenesis of non-exudative age-related macular degeneration.
为了确定暴露于蓝光发光二极管（LED）光下导致的色素性小鼠视网膜色素上皮（RPE）和光感受器损伤的特征，并确定引起损伤的机制。暴露于蓝光 LED 光 3 天可诱导视网膜损伤，其损伤特征与暴露于白色荧光灯不同。检眼镜检查显示，暴露于蓝光 LED 3 天可导致视网膜上出现白点，组织学检查显示光感受器的 IS/OS 交界处有物质堆积。堆积的物质被离子钙结合衔接分子-1（Iba-1）染色，这是巨噬细胞的标志物。这些碎片也对过碘酸-Schiff（PAS）呈阳性。在蓝光 LED 暴露后不久，尤其是在视神经周围，RPE 区域扩大，导致光感受器的继发性变性。将色素性小鼠连续 3 天暴露于蓝光 LED 下会导致 RPE 和光感受器损伤。损伤导致巨噬细胞和类 drusen 物质在外节光感受器周围堆积。这种蓝光暴露模型可能有助于研究非渗出性年龄相关性黄斑变性的发病机制。
