Nakamura Maho, Kuse Yoshiki, Tsuruma Kazuhiro, Shimazawa Masamitsu, Hara Hideaki
Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University.
Biol Pharm Bull. 2017;40(8):1219-1225. doi: 10.1248/bpb.b16-01008.
The aim of study was to establish a mouse model of blue light emitting diode (LED) light-induced retinal damage and to evaluate the effects of the antioxidant N-acetylcysteine (NAC). Mice were exposed to 400 or 800 lx blue LED light for 2 h, and were evaluated for retinal damage 5 d later by electroretinogram amplitude and outer nuclear layer (ONL) thickness. Additionally, we investigated the effect of blue LED light exposure on shorts-wave-sensitive opsin (S-opsin), and rhodopsin expression by immunohistochemistry. Blue LED light induced light intensity dependent retinal damage and led to collapse of S-opsin and altered rhodopsin localization from inner and outer segments to ONL. Conversely, NAC administered at 100 or 250 mg/kg intraperitoneally twice a day, before dark adaptation and before light exposure. NAC protected the blue LED light-induced retinal damage in a dose-dependent manner. Further, blue LED light-induced decreasing of S-opsin levels and altered rhodopsin localization, which were suppressed by NAC. We established a mouse model of blue LED light-induced retinal damage and these findings indicated that oxidative stress was partially involved in blue LED light-induced retinal damage.
本研究的目的是建立蓝光发光二极管（LED）光诱导视网膜损伤的小鼠模型，并评估抗氧化剂N-乙酰半胱氨酸（NAC）的作用。将小鼠暴露于400或800勒克斯的蓝光LED光下2小时，并在5天后通过视网膜电图振幅和外核层（ONL）厚度评估视网膜损伤情况。此外，我们通过免疫组织化学研究了蓝光LED光暴露对短波敏感视蛋白（S-视蛋白）和视紫红质表达的影响。蓝光LED光诱导了光强度依赖性视网膜损伤，并导致S-视蛋白的崩解以及视紫红质的定位从内外节转移到ONL。相反，在暗适应前和光照前，每天两次腹腔注射100或250毫克/千克的NAC。NAC以剂量依赖性方式保护蓝光LED光诱导的视网膜损伤。此外，蓝光LED光诱导的S-视蛋白水平降低和视紫红质定位改变，均被NAC抑制。我们建立了蓝光LED光诱导视网膜损伤的小鼠模型，这些发现表明氧化应激部分参与了蓝光LED光诱导的视网膜损伤。
