Department of Laboratory Medicine, Shanghai General Hospital, Shanghai JiaoTong University School of Medicine, 100 Haining Road, Shanghai, China.
Department of Laboratory Medicine, Shanghai General Hospital, Shanghai JiaoTong University School of Medicine, 100 Haining Road, Shanghai, China.
Int J Pharm. 2018 Oct 5;549(1-2):115-123. doi: 10.1016/j.ijpharm.2018.07.047. Epub 2018 Jul 21.
Allergen specific immunotherapy (SIT) is the only specific therapeutic way for house dust mite (HDM) allergy. To improve the efficacy of SIT, hollow mesoporous silica nanoparticles (HMSNs) were used as vehicles for HDM allergen. The HMSNs were prepared and characterized. The major HDM allergen (Der f2) was loaded onto HMSNs, and the drug loading capacity and release profile were determined. Then the Der f2 loaded HMSNs were injected subcutaneously to mouse model of Der f2 induced allergic asthma and the preventive effects were evaluated. Our results showed that HMSNs were spherical (100 nm) with pore diameter of 2.897 nm and successfully loaded with Der f2 protein. The loading capacity is 90 μg Der f2/1 mg HMSNs. The Der f2 loaded on HMSNs released slowly in 72 h. Treatment with Der f2 loaded HMSNs could efficiently decrease Der f2 specific IgE levels, inflammatory cells infiltration in lung tissue, and Th2 cytokine IL4 levels in BALF. In the meanwhile, it could increase the Der f2 specific IgG levels, Th1 cytokine IFN-γ levels, and induce proliferation of splenocytes to Der f2 accompanied by increased IFN-γ levels. These results showed that Der f2 loaded HMSNs were efficient in preventing allergic inflammation, and HMSNs may be potential vehicles for SIT of HDM allergy.
变应原特异性免疫治疗（SIT）是治疗屋尘螨（HDM）过敏的唯一特异性治疗方法。为了提高 SIT 的疗效，中空介孔硅纳米粒子（HMSNs）被用作 HDM 过敏原的载体。制备并表征了 HMSNs。将主要的 HDM 过敏原（Der f2）加载到 HMSNs 上，并确定了载药量和释放曲线。然后将负载 Der f2 的 HMSNs 注射到 Der f2 诱导的过敏性哮喘小鼠模型中，评估其预防效果。我们的结果表明，HMSNs 呈球形（100nm），孔径为 2.897nm，并成功负载了 Der f2 蛋白。载药量为 90μg Der f2/1mg HMSNs。负载在 HMSNs 上的 Der f2 在 72 小时内缓慢释放。负载 Der f2 的 HMSNs 治疗可有效降低 Der f2 特异性 IgE 水平、肺组织中炎性细胞浸润和 BALF 中的 Th2 细胞因子 IL4 水平。同时，它可以增加 Der f2 特异性 IgG 水平、Th1 细胞因子 IFN-γ 水平，并诱导脾细胞对 Der f2 的增殖，同时伴有 IFN-γ 水平的增加。这些结果表明，负载 Der f2 的 HMSNs 能有效预防过敏炎症，HMSNs 可能是 HDM 过敏 SIT 的潜在载体。
