Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China.
Institute of Animal Husbandry and Veterinary Medicine, Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, China.
Front Immunol. 2021 Apr 19;12:644396. doi: 10.3389/fimmu.2021.644396. eCollection 2021.
The development of effective vaccines and delivery systems in aquaculture is a long-term challenge for controlling emerging and reemerging infections. Cost-efficient and advanced nanoparticle vaccines are of tremendous applicability in prevention of infectious diseases of fish. In this study, dihydrolipoamide dehydrogenase (DLDH) antigens of were loaded into mesoporous silica nanoparticles (MSN) to compose the vaccine delivery system. Hydroxypropyl methylcellulose phthalate (HP55) was coated to provide protection of immunogen. The morphology, loading capacity, acid-base triggered release were characterized and the toxicity of nanoparticle vaccine was determined . Further, the vaccine immune effects were evaluated in large yellow croaker oral administration. studies confirmed that the antigen could be stable in enzymes-rich artificial gastric fluid and released under artificial intestinal fluid environment. cytotoxicity assessment demonstrated the vaccines within 120 μg/ml have good biocompatibility for large yellow croaker kidney cells. Our data confirmed that the nanoparticle vaccine could elicit innate and adaptive immune response, and provide good protection against challenge. The MSN delivery system prepared may be a potential candidate carrier for fish vaccine oral administration feeding. Further, we provide theoretical basis for developing convenient, high-performance, and cost-efficient vaccine against infectious diseases in aquaculture.
在水产养殖中开发有效的疫苗和输送系统是控制新出现和重新出现的感染的长期挑战。具有成本效益和先进的纳米颗粒疫苗在预防鱼类传染病方面具有巨大的适用性。在这项研究中，将二氢硫辛酸脱氢酶 (DLDH) 抗原加载到介孔硅纳米颗粒 (MSN) 中，组成疫苗输送系统。用羟丙基甲基纤维素邻苯二甲酸酯 (HP55) 进行涂层，以提供免疫原的保护。对形态、载药量、酸碱触发释放进行了表征，并测定了纳米颗粒疫苗的毒性。进一步，通过大黄鱼口服途径评估了疫苗的免疫效果。研究证实，抗原在富含酶的人工胃液中稳定，并在人工肠液环境下释放。细胞毒性评估表明，疫苗在 120μg/ml 以内对大黄鱼肾细胞具有良好的生物相容性。我们的数据证实，纳米颗粒疫苗可以引发先天和适应性免疫反应，并为 提供良好的保护。所制备的 MSN 输送系统可能是鱼类疫苗口服途径的潜在候选载体。此外，我们为开发针对水产养殖中传染病的方便、高性能和具有成本效益的疫苗提供了理论依据。
