Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan; Center of Advanced Technology for the Environment, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Center of Advanced Technology for the Environment, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Mar Pollut Bull. 2018 Aug;133:773-780. doi: 10.1016/j.marpolbul.2018.06.014. Epub 2018 Jun 22.
To elucidate the effects of the herbicide Irgarol 1051 on the gene expression of heat shock protein 90 (HSP90) in hard corals, we isolated a full-length cDNA encoding HSP90 from Acropora tenuis, which has a deduced open reading frame of 732-amino acid residues with a predicted molecular mass of 84.5 kDa. The amino acid sequence of A. tenuis HSP90 showed a high degree of similarity with the hermatypic-coral HSP90 families. After a 7-d exposure to 1 or 10 μg/L of Irgarol, the body colours of corals in the 10 μg/L treatment group were significantly whiter (bleached), whereas no such effects were observed in the corals in the 1 μg/L treatment group. However, the expression level of coral HSP90 was significantly downregulated after exposure to both 1 and 10 μg/L Irgarol. These results suggest that A. tenuis HSP90 may be a useful molecular biomarker to predict bleaching caused by herbicides.
为了阐明除草剂 Irgarol 1051 对硬珊瑚热休克蛋白 90（HSP90）基因表达的影响，我们从软珊瑚 Acropora tenuis 中分离出一个 HSP90 的全长 cDNA，其开放阅读框为 732 个氨基酸残基，预测分子量为 84.5 kDa。A. tenuis HSP90 的氨基酸序列与有孔虫 HSP90 家族具有高度相似性。在暴露于 1 或 10μg/L Irgarol 7 天后，10μg/L 处理组珊瑚的体色明显变白（白化），而 1μg/L 处理组珊瑚则没有观察到这种影响。然而，暴露于 1 和 10μg/L Irgarol 后，珊瑚 HSP90 的表达水平显著下调。这些结果表明，A. tenuis HSP90 可能是预测除草剂引起白化的有用分子生物标志物。
