Central Research Laboratory, Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita, Hamamatsu, Shizuoka 434-8601, Japan.
Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan; Center of Advanced Technology for the Environment, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Mar Pollut Bull. 2017 Nov 30;124(2):687-693. doi: 10.1016/j.marpolbul.2017.08.006. Epub 2017 Sep 1.
We examined the effect of two herbicides (Irgarol 1051 and Diuron) on symbiotic dinoflagellates in the hard coral Acropora digitifera using delayed fluorescence (DF), specifically assessing changes in molecular membrane transport, i.e. inflow and outflow rates, and the binding of the herbicides to target proteins in photosystem II. The DF approach is rapid (e.g. measurement time, 60 s) and non-invasive, and can provide data on the extent of a photosynthetic system and the activity of its electron carriers. The DF of A. digitifera is inhibited 2 h after exposure to 1 μg/L of either Irgarol or Diuron. Analysis of DF inhibition over time by a compartment model suggests that Irgarol exposure results in a relatively higher inflow rate and lower outflow rate than does Diuron exposure. This suggests that Irgarol exposure more strongly inhibits photosynthesis and that the coral symbiotic dinoflagellates recover less from inhibition.
我们使用延迟荧光（DF）技术研究了两种除草剂（Irgarol 1051 和 Diuron）对硬珊瑚 Acropora digitifera 共生甲藻的影响，具体评估了分子膜转运的变化，即流入和流出速率，以及除草剂与光合作用系统 II 中靶蛋白的结合。DF 方法快速（例如，测量时间为 60 秒）且非侵入性，可以提供有关光合作用系统的程度及其电子载体活性的数据。暴露于 1μg/L 的 Irgarol 或 Diuron 2 小时后，A. digitifera 的 DF 受到抑制。通过隔室模型对 DF 抑制随时间的分析表明，与 Diuron 暴露相比，Irgarol 暴露导致相对较高的流入率和较低的流出率。这表明 Irgarol 暴露更强烈地抑制光合作用，珊瑚共生甲藻从抑制中恢复的程度较低。
