[Granular-cell tumor (Abrikosov). Immunohistological study of 4 cases with review of the literature].

Granular-cell tumour (GCT) is a benign neoplastic proliferation first described by Abrikossoff (1), who considered it to be of muscular origin. Since then, however, the histogenesis of GCT has been a matter of serious controversy, and various cell types have been considered as being the origin of GCT. In this work we investigated the immunohistochemical profile of four cases of GCT using antibodies to several neural differentiation markers: S-100 protein, neurofilaments (NF), glial fibrillary acidic protein (GFAP). The results were compared to those obtained on three cases of schwannomas (S) and to those already reported in literature. Four cases of GCT were retrieved from the files of the Laboratory of Histopathology of the Clinic of Dermatology, Hôp. E.-Herriot. The tumours had been observed during a two-year-period (1983-1984), fixed in Bouin's fixative and embedded in paraffin. In parallel, three cases of S that were treated in the same way were also studied. This was performed on 3 mu-thick paraffin sections using the avidin-biotin-peroxidase complex method (kit Vectastain, Vector Lab., Burlingame, USA). The following antibodies were used: a) antiserum to protein S-100 (Dakopatts, Denmark) (working dilution 1: 50); b) monoclonal antibody to 200 Kd NF (Labsystem, Helsinki) (working dilution 1: 40): C) monoclonal antibody to GFAP (Biosoft, Paris) (working dilution 1: 10).(ABSTRACT TRUNCATED AT 250 WORDS)