Brandt N, Bassett A
Arch Biochem Biophys. 1986 Feb 1;244(2):872-5. doi: 10.1016/0003-9861(86)90657-0.
When microsomes from feline ventricular muscle are centrifuged on continuous linear sucrose gradients, the major peak for the distribution pattern of the dihydropyridine binding sites corresponds in position and shape with the distribution of the Mr 300K polypeptide marker for junctional sarcoplasmic reticulum (SR). Plasma membrane vesicles are also present in those gradient fractions and appear to be joined to the junctional SR as native dyads. We now report that when such putative dyads are passed through the French press, both the dihydropyridine binding sites and the plasma membrane marker band together at a new isopycnic point distinct from the junctional SR. We conclude that as has been found in the skeletal muscle system the dihydropyridine binding sites are a marker for the junctional domain of the plasma membrane and that separation of the dyad components of the mammalian myocardium can be attained.
当将猫心室肌微粒体在连续线性蔗糖梯度上进行离心时，二氢吡啶结合位点分布模式的主要峰在位置和形状上与连接肌质网（SR）的Mr 300K多肽标志物的分布相对应。质膜囊泡也存在于那些梯度级分中，并且似乎以天然二联体的形式与连接SR相连。我们现在报告，当将这种假定的二联体通过法国压榨机时，二氢吡啶结合位点和质膜标志物带会在一个不同于连接SR的新等密度点处一起出现。我们得出结论，正如在骨骼肌系统中所发现的那样，二氢吡啶结合位点是质膜连接结构域的标志物，并且可以实现哺乳动物心肌二联体成分的分离。
