Benjamino Jacquelynn, Beka Lidia, Graf Joerg
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut.
Curr Protoc Toxicol. 2018 Aug;77(1):e53. doi: 10.1002/cptx.53. Epub 2018 Jul 25.
Microbiomes can be thought of as the integration of biotic and abiotic factors, including the microbial communities and the distinct physio-chemical properties that are present in a habitat. The microbes within a microbiome can influence the chemical environment by degrading medications inside patients or contaminants in the environment, such as hydrocarbons released after the Deepwater Horizon oil spill. Identifying and monitoring the relative abundance of microbes can help to elucidate variations in their response to toxins and should be considered as a variable in statistical analyses. In addition, the presence of pathogenic microbes or toxin-producing species could affect the observed toxicity of an environment. The protocols in this unit begin with the collection of samples, proceeds to sequencing of the bacterial DNA, and ends with an analysis of the bacterial community in light of environmental metadata. © 2018 by John Wiley & Sons, Inc.
微生物群落可以被看作是生物和非生物因素的整合，包括微生物群落以及栖息地中存在的独特理化性质。微生物群落中的微生物可以通过降解患者体内的药物或环境中的污染物（如深水地平线石油泄漏后释放的碳氢化合物）来影响化学环境。识别和监测微生物的相对丰度有助于阐明它们对毒素反应的差异，并且在统计分析中应将其视为一个变量。此外，致病微生物或产毒素物种的存在可能会影响所观察到的环境毒性。本单元中的实验方案始于样本采集，接着是细菌DNA测序，最后根据环境元数据对细菌群落进行分析。© 2018约翰威立父子出版公司
