醛缩酶A信使核糖核酸在人类成人及胎儿组织以及肝癌中的表达。
Expression of aldolase A messenger RNAs in human adult and foetal tissues and in hepatoma.
作者信息
Mennecier F, Daegelen D, Schweighoffer F, Levin M, Kahn A
出版信息
Biochem Biophys Res Commun. 1986 Feb 13;134(3):1093-100. doi: 10.1016/0006-291x(86)90363-3.
3 specific cDNA clones for human aldolase A were isolated from a human muscle library. One of them was subcloned in M 13 phage, then used as a probe to investigate the patterns and the levels of aldolase A mRNA in various human tissues. Two mRNA species differing in length were observed. The lighter one -1550 bases- was found specific to skeletal muscle; its amount increased during muscle development. The heavier aldolase A mRNA -1650 bases- accounted for foetal and ubiquitous presence of aldolase A isozyme. The resurgence of aldolase A in hepatomas occurred through this latter mRNA species.
从人肌肉文库中分离出3个人醛缩酶A的特异性cDNA克隆。其中一个被亚克隆到M13噬菌体中，然后用作探针来研究醛缩酶A mRNA在各种人体组织中的模式和水平。观察到两种长度不同的mRNA种类。较轻的一种——1550个碱基——被发现是骨骼肌特有的；其数量在肌肉发育过程中增加。较重的醛缩酶A mRNA——1650个碱基——解释了醛缩酶A同工酶在胎儿期的普遍存在。肝癌中醛缩酶A的再现是通过后一种mRNA种类发生的。
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