Mukai T, Joh K, Miyahara H, Sakakibara M, Arai Y, Hori K
Biochem Biophys Res Commun. 1984 Mar 15;119(2):575-81. doi: 10.1016/s0006-291x(84)80287-9.
Rat aldolase A mRNAs from the skeletal muscle and ascites hepatoma cells are compared by RNA blot hybridization. The hepatoma AH60C and AH130 mRNA are about 1600 bases long, some 50 bases longer than the skeletal muscle transcript. Aldolase A enzymes encoded by these mRNAs, however, are indistinguishable in electrophoresis on cellulose polyacetate strips and two-dimensional polyacrylamide gel. In addition, comparison of the thermal stability of hybrids between the mRNAs from the skeletal muscle and hepatoma cells and a cDNA probe (pRAAM83) made from the aldolase A mRNA of the skeletal muscle indicate that the mRNAs have extremely high sequence homology, at least, within the most part of coding region in addition to the 3' noncoding region. These findings indicate that these mRNA may be transcribed by the use of different promoter sites and the common structural gene.
通过RNA印迹杂交比较了来自骨骼肌和腹水肝癌细胞的大鼠醛缩酶A mRNA。肝癌AH60C和AH130的mRNA约1600个碱基长，比骨骼肌转录本长约50个碱基。然而，由这些mRNA编码的醛缩酶A酶在醋酸纤维素条和二维聚丙烯酰胺凝胶上的电泳中无法区分。此外，对骨骼肌和肝癌细胞的mRNA与由骨骼肌醛缩酶A mRNA制备的cDNA探针（pRAAM83）之间杂交体的热稳定性比较表明，这些mRNA除了3'非编码区外，在编码区的大部分区域也具有极高的序列同源性。这些发现表明，这些mRNA可能是通过使用不同的启动子位点和共同的结构基因转录而来的。
