Department of Ophthalmology, National Defense Medical College, Tokorozawa, Saitama, Japan.
Department of Developmental Anatomy and Regenerative Biology, National Defense Medical College, Tokorozawa, Saitama, Japan.
Oxid Med Cell Longev. 2018 Jun 27;2018:6065285. doi: 10.1155/2018/6065285. eCollection 2018.
Indocyanine green (ICG) angiography is an indispensable inspection to diagnose and treat for chorioretinal diseases. In this study, we investigated the phototoxicity of ICG on RPE cells at the levels of residual ICG after angiography under ambient light. After incubation of ARPE-19 cells in a colorless medium containing 0 to 10 g/mL ICG for 24 hours in the dark or under 2000 lx illumination from a fluorescent lamp, cell viability decreased and cell death rate increased in cultures with more than 5.0 g/mL ICG under illumination. In culture with 10 g/mL ICG under illumination, morphology of cells changed to be oval and TUNEL- and malondialdehyde-positive cells increased compared to other cultures with ICG in the dark or without ICG under illumination. Furthermore, the level of intracellular reactive oxygen species was also elevated. On the other hand, toxicity of ICG denatured by illumination was not observed. Blocking green to red light overlapping wavelengths of ICG absorbance exhibited decreased cell death rate. The present study indicated that ICG at the estimated intravenous concentrations after ICG angiography induces potential phototoxicity on human RPE cells via oxidative damage under continuous ambient illumination and that the cytotoxicity is reduced by blocking green to red light wavelengths.
吲哚菁绿（ICG）血管造影术是诊断和治疗脉络膜视网膜疾病不可或缺的检查手段。在这项研究中，我们研究了在环境光下血管造影后残留 ICG 水平下 ICG 对 RPE 细胞的光毒性。将 ARPE-19 细胞在黑暗中或在 2000 lx 荧光灯照射下于含 0 至 10 g/mL ICG 的无色培养基中孵育 24 小时后，在光照下，ICG 浓度超过 5.0 g/mL 的培养物中的细胞活力降低，细胞死亡率增加。在 10 g/mL ICG 光照下培养时，与黑暗中含 ICG 或光照下不含 ICG 的其他培养物相比，细胞形态变为椭圆形，TUNEL 和丙二醛阳性细胞增加。此外，细胞内活性氧水平也升高。另一方面，光照下变性的 ICG 没有表现出毒性。阻断与 ICG 吸收重叠的绿光到红光波长可降低细胞死亡率。本研究表明，血管造影后估计静脉内浓度的 ICG 通过持续环境光照下的氧化损伤对人 RPE 细胞产生潜在的光毒性，并且通过阻断绿光到红光波长可降低细胞毒性。
