Cozzolino M, Guarino F, Castiglione S, Cicatelli A, Celia G
Department of Human, Philosophical and Educational Sciences, University of Salerno, Italy.
Department of Chemistry and Biology, University of Salerno, Italy.
Transl Med UniSa. 2018 Mar 31;17:37-41. eCollection 2017 Jul.
In the last years, epigenetics and functional genomics methods to evaluate the genomic effects and mechanisms of mind-body therapies have increasingly grown. DNA microarray technology has been used to show the involvement of the stress response pathways both in the case of disease and stress and as an effect of mind-body therapies. In the present research, the DNA samples obtained from 20 individuals who experienced a mind-body therapeutic protocol (MBT-T), were analysed from the bio-molecular point of view by means of an epigenetic marker (MSAP molecular tool), in order to estimate the different status of methylation. The subjects were compared at 3 different times: prior to, 1 hour after, and 24 hours after the treatment. The molecular data were processed through different biostatistics approaches: the Bayesian statistics approach, in order to estimate the clustering membership of the subjects (Structure), and the statistical estimation of the DNA methylation level (MSAP statistical tool). The structure analysis revealed that the clusters and their membership changed among the three time points moving from higher heterogeneous distribution to higher homogeneous clusters. Before the treatment, the subjects' epigenetic profiles were heterogeneous; after the mind-body treatment we found that epigenetic profiles converged to homogeneous DNA methylation status. DNA epigenetic status of the subjects was affected by the MBT-T treatment.
在过去几年中，用于评估身心疗法的基因组效应和机制的表观遗传学和功能基因组学方法日益增多。DNA微阵列技术已被用于表明应激反应途径在疾病和应激情况下的参与情况，以及作为身心疗法的一种效应。在本研究中，从20名经历了身心治疗方案（MBT-T）的个体中获取的DNA样本，通过一种表观遗传标记（MSAP分子工具）从生物分子角度进行了分析，以估计甲基化的不同状态。在3个不同时间点对受试者进行了比较：治疗前、治疗后1小时和治疗后24小时。分子数据通过不同的生物统计学方法进行处理：贝叶斯统计方法，以估计受试者的聚类成员身份（Structure），以及DNA甲基化水平的统计估计（MSAP统计工具）。结构分析表明，聚类及其成员身份在三个时间点之间发生了变化，从较高的异质分布转变为较高的同质聚类。治疗前，受试者的表观遗传谱是异质的；身心治疗后，我们发现表观遗传谱收敛到同质的DNA甲基化状态。受试者的DNA表观遗传状态受到MBT-T治疗的影响。
