Haribabu B, Rajkovic A, Dottin R P
Dev Biol. 1986 Feb;113(2):436-42. doi: 10.1016/0012-1606(86)90178-8.
UDP glucose pyrophosphorylase (UDPGP) (EC.2.7.7.9) is a developmentally regulated enzyme of Dictyostelium discoideum. Two polypeptides of UDPGP are translated from Dictyostelium mRNA. Recently we isolated a cDNA clone which encodes one of the UDPGP polypeptides (B. R. Fishel, J. A. Ragheb, A. Rajkovic, B. Haribabu, C. W. Schweinfest, and R. P. Dottin (1985). Dev. Biol. 110, 369-381). By hybridization with the cDNA and by in vitro translation and immunoprecipitation, we examined the effect of cell-cell contact and cAMP on the regulation of UDPGP expression. Disaggregation of slugs resulted in a rapid loss of UDPGP mRNA. Addition of cAMP to these cells resulted in increased levels of UDPGP mRNA, though not to the same extent as seen during normal development. The two UDPGP polypeptides observed in vitro are coordinately regulated. Unaggregated cells, starved and shaken rapidly in suspension, did not show UDPGP mRNA accumulation. However, addition of cAMP to these cells caused UDPGP induction, suggesting that the requirement for cell-cell contact could be bypassed in part by cAMP addition.
尿苷二磷酸葡萄糖焦磷酸化酶（UDPGP）（EC.2.7.7.9）是盘基网柄菌中一种受发育调控的酶。UDPGP的两种多肽由盘基网柄菌的信使核糖核酸翻译而来。最近我们分离出了一个编码UDPGP多肽之一的cDNA克隆（B.R.菲舍尔、J.A.拉格赫布、A.拉伊科维奇、B.哈里巴布、C.W.施温费斯特和R.P.多廷（1985年）。《发育生物学》110卷，第369 - 381页）。通过与该cDNA杂交以及体外翻译和免疫沉淀，我们研究了细胞间接触和环磷酸腺苷（cAMP）对UDPGP表达调控的影响。蛞蝓体的解体导致UDPGP信使核糖核酸迅速减少。向这些细胞中添加cAMP会使UDPGP信使核糖核酸水平升高，尽管升高幅度不如正常发育过程中那么大。体外观察到的两种UDPGP多肽受到协同调控。未聚集的细胞在悬浮状态下饥饿并快速振荡，未显示出UDPGP信使核糖核酸的积累。然而，向这些细胞中添加cAMP会诱导UDPGP的产生，这表明细胞间接触的需求可以通过添加cAMP部分绕过。
