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BP74的表达与组织，BP74是一种在盘基网柄菌发育过程中表达的环磷酸腺苷调节基因。

Expression and organization of BP74, a cyclic AMP-regulated gene expressed during Dictyostelium discoideum development.

作者信息

Hopkinson S B, Pollenz R S, Drummond I, Chisholm R L

机构信息

Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, Illinois 60611.

出版信息

Mol Cell Biol. 1989 Oct;9(10):4170-8. doi: 10.1128/mcb.9.10.4170-4178.1989.

DOI:10.1128/mcb.9.10.4170-4178.1989
PMID:2555685
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362495/
Abstract

We have characterized a cDNA and the corresponding gene for a cyclic AMP-inducible gene expressed during Dictyostelium development. This gene, BP74, was found to be first expressed about the time of aggregate formation, approximately 6 h after starvation. Accumulation of BP74 mRNA did not occur in Dictyostelium cells that had been starved in fast-shaken suspension cultures but was induced in similar cultures to which cyclic AMP pulses had been added. The BP74 cDNA and gene were characterized by DNA sequence analysis and transcriptional mapping. When the BP74 promoter region was fused with a chloramphenicol acetyltransferase reporter gene and reintroduced into Dictyostelium cells, the transfected chloramphenicol acetyltransferase gene displayed the same developmentally regulated pattern of expression as did the endogenous BP74 gene, suggesting that all of the cis-acting elements required for regulated expression were carried by a 2-kilobase cloned genomic fragment. On the basis of sequence analysis, the gene appeared to encode a protein containing a 20-residue hydrophobic sequence at the amino-terminal end and 26 copies of a 20-amino-acid repeat.

摘要

我们已经鉴定出一种在盘基网柄菌发育过程中表达的环磷酸腺苷（cAMP）诱导基因的互补DNA（cDNA）及相应基因。这个名为BP74的基因在饥饿约6小时后，即聚集形成时开始首次表达。在快速振荡悬浮培养中饥饿的盘基网柄菌细胞中，BP74信使核糖核酸（mRNA）并未积累，但在添加了cAMP脉冲的类似培养物中被诱导表达。通过DNA序列分析和转录图谱对BP74 cDNA和基因进行了鉴定。当BP74启动子区域与氯霉素乙酰转移酶报告基因融合并重新导入盘基网柄菌细胞时，转染的氯霉素乙酰转移酶基因显示出与内源性BP74基因相同的发育调控表达模式，这表明调控表达所需的所有顺式作用元件都由一个2千碱基的克隆基因组片段携带。基于序列分析，该基因似乎编码一种蛋白质，其在氨基末端含有一个20个残基的疏水序列和26个20氨基酸重复序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/1a73636072d3/molcellb00058-0068-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/83414651cf7a/molcellb00058-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/acce70729152/molcellb00058-0064-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/0053551117d6/molcellb00058-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/915c80bb4968/molcellb00058-0065-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/1605c892607d/molcellb00058-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/1a73636072d3/molcellb00058-0068-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/83414651cf7a/molcellb00058-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/acce70729152/molcellb00058-0064-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/0053551117d6/molcellb00058-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/915c80bb4968/molcellb00058-0065-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/1605c892607d/molcellb00058-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad6b/362495/1a73636072d3/molcellb00058-0068-b.jpg

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