Key Laboratory of Exercise and Health Sciences, Shanghai University of Sport, Shanghai, China.
College of Science, Donghua University, Shanghai, China.
Biol Reprod. 2019 Jan 1;100(1):133-138. doi: 10.1093/biolre/ioy170.
Benign prostatic hyperplasia (BPH) develops more likely with increasing age and changing serum concentrations of circulating estradiol (E2) and/or testosterone (T). In this study, we explored the relationship between serum E2/T ratio and BPH risk in rats by fitting a mathematical model. A total of 176 rats were randomized to one of the following treatment groups: normal control, castrated control, and 20 more groups of castrated animals treated with increasing dose combinations of T and E2, once daily for 30 days. Serial blood samples were obtained to determine serum T and E2 levels by magnetic bead enzyme-linked immunosorbent assay. Prostate tissue was taken to measure prostate volume. MATLAB software was used to simulate the relationship between prostate/body weight ratio (PBR) and E2/T ratio with a mathematical equation. The values of PBR, E2 and T in the treatment groups were significantly higher than those in the control groups. Stepwise regression showed that PBR was a function of E2 and T. PBR = -0.1782 + 0.0081 E2 + 0.063 T - 0.6 × 10-5 E22 - 0.28 × 10-3 T2. E2/T ratio change may be one of the risk factors for PBR, which is associated with the development of BPH.
良性前列腺增生症（BPH）的发生更可能随着年龄的增长和循环雌二醇（E2）和/或睾酮（T）血清浓度的变化而发生。在这项研究中，我们通过拟合数学模型来探讨血清 E2/T 比值与大鼠 BPH 风险之间的关系。将 176 只大鼠随机分为以下治疗组之一：正常对照组、去势对照组和 20 个去势动物治疗组，每天用不同剂量的 T 和 E2 组合处理一次，共 30 天。连续采集血样，用磁珠酶联免疫吸附法测定血清 T 和 E2 水平。取前列腺组织测量前列腺体积。使用 MATLAB 软件，用数学方程模拟前列腺/体重比（PBR）与 E2/T 比值之间的关系。治疗组的 PBR、E2 和 T 值明显高于对照组。逐步回归显示 PBR 是 E2 和 T 的函数。PBR = -0.1782 + 0.0081 E2 + 0.063 T - 0.6×10-5 E22 - 0.28×10-3 T2。E2/T 比值的变化可能是 PBR 的危险因素之一，与 BPH 的发生有关。
