Zaia J A, Forman S J, Ting Y P, Vanderwal-Urbina E, Blume K G
J Infect Dis. 1986 Apr;153(4):780-7. doi: 10.1093/infdis/153.4.780.
Human cytomegalovirus (HCMV) infection continues to be the most important infection occurring after allogeneic bone marrow transplantation (BMT). Although T cell-specific antiviral immunity appears to be necessary for control of the infection, the humoral immune reaction also contributes to a complete immune response. In this paper we report our findings concerning the antibody response to the individual polypeptides of HCMV in patients who had undergone BMT and subsequently had displayed evidence of HCMV infection. Sera obtained from the subjects during and after HCMV viremia were studied by using both a radioimmunoprecipitation assay and an immunoblot assay, and the results were compared with the pattern of antibody response in normal individuals. The results show that the BMT patient can make antibodies to individual proteins of HCMV in a pattern similar to that displayed by persons with natural infection. The polypeptide-specific antibody response present most frequently was directed to proteins of 64 kDa, 50 kDa, and 36 kDa. Some BMT recipients also produced antibody to a wide range of HCMV proteins: 183 kDa, 155 kDa, 130 kDa, 110 kDa, 92 kDa, 86 kDa, 74 kDa, 71 kDa, 69 kDa, 39-44 kDa, 33 kDa, and 29 kDa.
人巨细胞病毒（HCMV）感染仍然是异基因骨髓移植（BMT）后发生的最重要的感染。尽管T细胞特异性抗病毒免疫似乎是控制感染所必需的，但体液免疫反应也有助于形成完整的免疫应答。在本文中，我们报告了关于接受BMT并随后出现HCMV感染证据的患者对HCMV各个多肽的抗体反应的研究结果。通过放射免疫沉淀试验和免疫印迹试验对受试者在HCMV病毒血症期间及之后获得的血清进行了研究，并将结果与正常个体的抗体反应模式进行了比较。结果表明，BMT患者能够以与自然感染个体相似的模式产生针对HCMV各个蛋白质的抗体。最常出现的多肽特异性抗体反应针对的是64 kDa、50 kDa和36 kDa的蛋白质。一些BMT受者还产生了针对多种HCMV蛋白质的抗体：183 kDa、155 kDa、130 kDa、110 kDa、92 kDa、86 kDa、74 kDa、71 kDa、69 kDa、39 - 44 kDa、33 kDa和29 kDa。
