Laboratory of Nanomaterials, Chang Gung University, Taoyuan, Taiwan.
Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan, Taiwan.
Sci Rep. 2018 Jul 27;8(1):11310. doi: 10.1038/s41598-018-29573-6.
Mineral nanoparticles form in physiological and pathological processes occurring in the human body. The calcium phosphate mineral phase of the particles has affinity for proteins and lipids, but the complete profiling of the organic molecules that bind to the particles has not been described in detail. We report here a comprehensive analysis of organic components found in mineralo-organic particles derived from body fluids. Based on biological staining, fluorescent tagging, proteomics and metabolomics, our results indicate that the mineral particles bind to proteins, amino acids, carbohydrates, polysaccharides, phospholipids, fatty acids, DNA and low molecular weight metabolites. These results can be used to study the formation and effects of mineralo-organic particles in biological fluids.
矿物质纳米颗粒在人体生理和病理过程中形成。颗粒的磷酸钙矿物质相与蛋白质和脂质具有亲和力，但与颗粒结合的有机分子的完整分析尚未详细描述。我们在此报告了对源自体液的矿化-有机颗粒中发现的有机成分的全面分析。基于生物染色、荧光标记、蛋白质组学和代谢组学，我们的结果表明，矿物质颗粒与蛋白质、氨基酸、碳水化合物、多糖、磷脂、脂肪酸、DNA 和低分子量代谢物结合。这些结果可用于研究生物液中矿化-有机颗粒的形成和作用。
