Senchukova Marina, Tomchuk Olesya, Shurygina Elena, Letuta Sergey, Alidzhanov Eskender, Nikiyan Hike, Razdobreev Dmitry
Department of Oncology, Orenburg State Medical University, Orenburg 460000, Russia.
Department of Histology, Cytology, Embryology, Orenburg State Medical University, Orenburg 460000, Russia.
Biomedicines. 2019 Mar 19;7(1):21. doi: 10.3390/biomedicines7010021.
Previously, we have shown the possibility of intramucosal gastric carcinoma induction by the intragastric administration of a mixture of formaldehyde and hydrogen peroxide in rats. In this study, we report a sizable increase in carcinogenic properties of the mixture when a suspension containing calcium carbonate nanoparticles was added to it. This technique allowed us to reduce both the number of the carcinogen administrations from twelve to two and the time to the cancer induction from six to four months. Although the induced tumors were represented by the intramucosal carcinomas, they were characterized by the extensive invasion of individual tumor cells and their clusters into the muscle layer and serosa as well as into the omentum and blood vessels. Considering that the invasive tumor cells were positive for vimentin, Snail and TGF-β2, we concluded that their invasion was the result of the activation of epithelial⁻mesenchymal transition (EMT) mechanisms. Thus, taking into account the data obtained, it can be assumed that under the conditions of inflammation or carcinogenesis, the calcium carbonate nanoparticles may affect the activation of EMT mechanisms.
此前，我们已经证明，通过在大鼠胃内给予甲醛和过氧化氢的混合物可诱发胃黏膜内癌。在本研究中，我们报告称，当向该混合物中添加含有碳酸钙纳米颗粒的悬浮液时，混合物的致癌特性显著增强。这项技术使我们能够将致癌物给药次数从12次减少到2次，并将癌症诱发时间从6个月缩短到4个月。尽管诱发的肿瘤以黏膜内癌为主，但它们的特征是单个肿瘤细胞及其细胞簇广泛侵入肌层、浆膜，以及大网膜和血管。鉴于侵袭性肿瘤细胞波形蛋白、Snail和转化生长因子-β2呈阳性，我们得出结论，它们的侵袭是上皮-间质转化（EMT）机制激活的结果。因此，考虑到所获得的数据，可以假设在炎症或致癌作用的条件下，碳酸钙纳米颗粒可能会影响EMT机制的激活。
