Chasey D, Banks J
J Gen Virol. 1986 Mar;67 ( Pt 3):567-76. doi: 10.1099/0022-1317-67-3-567.
Colostrum-deprived lambs experimentally infected with an atypical ovine rotavirus isolated from naturally scouring animals, and a naturally infected colostrum-deprived lamb, were examined by immunofluorescence and immunoperoxidase labelling, and electron microscopy. A hyperimmune serum to the virus was produced in a gnotobiotic lamb and used to demonstrate antigen in the villous epithelial cells of the small intestine from infected animals. Scanning and transmission electron microscopy of tissues from infected animals revealed giant multinucleate syncytia composed of fused epithelial cells. Infected cells contained intracytoplasmic virus particles which resembled group A rotaviruses in morphology and morphogenesis. Small numbers of infected cells in MA104 cultures inoculated with ovine atypical rotavirus could be detected by immunofluorescence but virus growth could not be maintained by passage. Virus particles were seen by thin-section electron microscopy but their morphology and morphogenesis were abnormal; they were each composed of a single electron-dense shell, with no core, and were associated with envelopes of smooth membrane rather than rough endoplasmic reticulum.
对初乳缺乏的羔羊进行了研究，这些羔羊通过实验感染了从自然腹泻动物中分离出的非典型绵羊轮状病毒，还有一只自然感染的初乳缺乏羔羊，采用免疫荧光和免疫过氧化物酶标记以及电子显微镜检查。在无菌羔羊中制备了针对该病毒的超免疫血清，并用于在受感染动物小肠的绒毛上皮细胞中显示抗原。对受感染动物组织的扫描电子显微镜和透射电子显微镜检查显示，由融合上皮细胞组成的巨大多核合胞体。受感染细胞含有胞质内病毒颗粒，其形态和形态发生类似于A组轮状病毒。用绵羊非典型轮状病毒接种的MA104培养物中少量受感染细胞可通过免疫荧光检测到，但病毒生长不能通过传代维持。通过超薄切片电子显微镜可以看到病毒颗粒，但其形态和形态发生异常；它们每个都由单个电子致密外壳组成，没有核心，并且与光滑膜的包膜相关，而不是粗糙内质网。
